Results 101 to 110 of about 24,718 (294)

Targeted Control of Gene Expression Using CRISPR-Associated Endoribonucleases

Cells
CRISPR-associated endoribonucleases (Cas RNases) cleave single-stranded RNA in a highly sequence-specific manner by recognizing and binding to short RNA sequences known as direct repeats (DRs).
Sagar J. Parikh, Heather M. Terron, Luke A. Burgard, Derek S. Maranan, Dylan D. Butler, Abigail Wiseman, Frank M. LaFerla, Shelley Lane, Malcolm A. Leissring   +8 more
doaj   +1 more source

Structural and Biochemical Characterization of Endoribonuclease Nsp15 Encoded by Middle East Respiratory Syndrome Coronavirus

Journal of Virology, 2018
The lethally pathogenic Middle East respiratory syndrome coronavirus (MERS-CoV) and the severe acute respiratory syndrome coronavirus (SARS-CoV) pose serious threats to humans.
Lianqi Zhang, Lei Li, Liming Yan, Z. Ming, Zhihui Jia, Z. Lou, Z. Rao   +6 more
semanticscholar   +1 more source

The Endoribonuclease RNase E Coordinates Expression of mRNAs and Small Regulatory RNAs and Is Critical for the Virulence of Brucella abortus

Journal of Bacteriology, 2020
Brucellosis is a debilitating disease of humans and animals globally, and there is currently no vaccine to combat human infection by Brucella spp. Moreover, effective antibiotic treatment in humans is extremely difficult and can lead to disease relapse ...
Lauren M Sheehan, James A. Budnick, Jaquille Fyffe-Blair, Kellie A. King, R. Settlage, Clayton C. Caswell   +5 more
semanticscholar   +1 more source

Protonated Glutamate and Aspartate Side Chains Can Recognize Phosphodiester Groups via Strong and Short Hydrogen Bonds in Biomacromolecular Complexes

Angewandte Chemie International Edition, Volume 64, Issue 27, July 1, 2025.
The highly negatively charged phosphodiester groups of nucleic acids and cyclic (di‐)nucleotides often interact with amino acids containing polar or positively charged sidechains. Due to charge repulsion with their negatively charged carboxylate groups in their side chains, glutamate and aspartate are considered to be detrimental to such interactions ...
Konstantin Neißner, Elke Duchardt‐Ferner, Christoph Wiedemann, Julian Kraus, Ute A. Hellmich, Jens Wöhnert   +5 more
wiley   +1 more source

It takes two (Las1 HEPN endoribonuclease domains) to cut RNA correctly

Journal of Biological Chemistry, 2020
The ribosome biogenesis factor Las1 is an essential endoribonuclease that is well-conserved across eukaryotes and a newly established member of the higher eukaryotes and prokaryotes nucleotide-binding (HEPN) domain-containing nuclease family.
M. Pillon, K. Goslen, J. Gordon, M. L. Wells, Jason G. Williams, R. Stanley   +5 more
semanticscholar   +1 more source

ESSENTIAL MEIOTIC ENDONUCLEASE 1 is required for chloroplast development and DNA repair in rice

Plant Biotechnology Journal, Volume 23, Issue 7, Page 2931-2948, July 2025.
Summary Chloroplast development is fundamental to photosynthesis and plant growth but is sensitive to environmental stress. Chloroplast development and division require genome stability and DNA repair, but the underlying mechanisms have been unclear. Using a forward genetic approach, we identified the striped‐leaf mutant k48 in the rice (Oryza sativa L.
Yanxin Du, Yang Li, Weijiang Tang, Weiping Mo, Tingting Ma, Rongcheng Lin   +5 more
wiley   +1 more source

Alkane biosynthesis gene expression and its increased production in recombinant cyanobacteria

FEBS Open Bio, Volume 15, Issue 6, Page 949-962, June 2025.
The transcription and mRNA structures of the alkane biosynthesis genes ado/aar were analysed in the representative cyanobacteria PCC 6803 and SK1‐2‐1, which produce C17H36 and C15H32, respectively. PCC 6803 transconjugants carrying these genes showed a 1.8‐ to 2.3‐fold increase in C17H36 production.
Misato Nagao, Takato Ozaki, Hirofumi Fukuda, Yu Kanesaki, Munehiko Asayama   +4 more
wiley   +1 more source

Downregulation of PIK3CA via antibody-esiRNA-complexes suppresses human xenograft tumor growth. [PDF]

PLoS ONE, 2018
Precision cancer therapy requires on the one hand detailed knowledge about a tumor's driver oncogenes and on the other hand an effective targeted therapy that specifically inhibits these oncogenes.
Nicole Bäumer, Jan Rehkämper, Neele Appel, Lisa Terheyden, Wolfgang Hartmann, Eva Wardelmann, Frank Buchholz, Carsten Müller-Tidow, Wolfgang E Berdel, Sebastian Bäumer   +9 more
doaj   +1 more source

RNA Recognition and Cleavage by the SARS Coronavirus Endoribonuclease

Journal of Molecular Biology, 2006
The emerging disease SARS is caused by a novel coronavirus that encodes several unusual RNA-processing enzymes, including non-structural protein 15 (Nsp15), a hexameric endoribonuclease that preferentially cleaves at uridine residues. How Nsp15 recognizes and cleaves RNA is not well understood and is the subject of this study.
Jingchuan Sun, Kanchan Bhardwaj, C. Cheng Kao, Linda A. Guarino, Andreas Holzenburg   +4 more
openaire   +3 more sources

Proteomic analysis of the excretory-secretory products from larval stages of Ascaris suum reveals high abundance of glycosyl hydrolases [PDF]

, 2013
Background: Ascaris lumbricoides and Ascaris suum are socioeconomically important and widespread parasites of humans and pigs, respectively. The excretory-secretory (ES) molecules produced and presented at the parasite-host interface during the different
Deforce, Dieter, Dhaenens, Maarten, Gasser, Robin B, Geldhof, Peter, Jex, Aaron R, Van Steendam, Katleen, Vlaminck, Johnny, Wang, Tao   +7 more
core   +5 more sources