DGR mutagenic transposition occurs via hypermutagenic reverse transcription primed by nicked template RNA. [PDF]
, 2017 Diversity-generating retroelements (DGRs) are molecular evolution machines that facilitate microbial adaptation to environmental changes. Hypervariation occurs via a mutagenic retrotransposition process from a template repeat (TR) to a variable repeat ...
Guo, Huatao +6 more
core +3 more sources
Defective Processing of Cytoplasmic and Chloroplast Ribosomal RNA in the Absence of Arabidopsis DXO1
Plant, Cell &Environment, Volume 48, Issue 6, Page 4227-4244, June 2025.ABSTRACT Decapping 5′‐3′ exoribonucleases from the DXO/Rai1 family are highly conserved among eukaryotes and exhibit diverse enzymatic activities depending on the organism. The biochemical and structural properties of the plant DXO1 differ from the yeast and animal counterparts, which is reflected in the in vivo functions of this enzyme.
Monika Zakrzewska‐Placzek +5 more
wiley +1 more source
YoeB-ribosome structure: a canonical RNase that requires the ribosome for its specific activity [PDF]
, 2017 As a typical endoribonuclease, YoeB mediates cellular adaptation in diverse bacteria by degrading mRNAs on its activation. Although the catalytic core of YoeB is thought to be identical to well-studied nucleases, this enzyme specifically targets mRNA ...
Chen, Yun +6 more
core
Phylogenetic identification of bacterial MazF toxin protein motifs among probiotic strains and foodborne pathogens and potential implications of engineered probiotic intervention in food [PDF]
, 2012 BACKGROUND: Toxin-antitoxin (TA) systems are commonly found in bacteria and Archaea, and it is the most common mechanism involved in bacterial programmed cell death or apoptosis. Recently, MazF, the toxin component of the toxin-antitoxin module, has been
Jing Hu +4 more
core +1 more source
The FASEB Journal, Volume 39, Issue 10, 31 May 2025.A proposed model showed that the RNA binding proteins MCPIP2 and IGF2BP1 competitively regulate the stability and expression of proangiogenic transcripts in tumor cells. MCPIP2 decays proangiogenic mRNAs by binding to the conserved stem–loop structure in the 3ʹ UTR via its RNase domain, resulting in inhibition of proangiogenic gene expression and ...
Wenbao Lu +4 more
wiley +1 more source
The role of ribonucleases in regulating global mRNA levels in the model organism Thermus thermophilus HB8 [PDF]
, 2014 BACKGROUND: RNA metabolism, including RNA synthesis and RNA degradation, is one of the most conserved biological systems and has been intensively studied; however, the degradation network of ribonucleases (RNases) and RNA substrates is not fully ...
Akeo Shinkai +7 more
core +1 more source
The physicist's guide to one of biotechnology's hottest new topics: CRISPR-Cas
, 2018 Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated proteins (Cas) constitute a multi-functional, constantly evolving immune system in bacteria and archaea cells.
Bonomo, Melia E., Deem, Michael W.
core +1 more source
Molecular mechanisms of CRISPR-mediated microbial immunity [PDF]
, 2013 Bacteriophages (phages) infect bacteria in order to replicate and burst out of the host, killing the cell, when reproduction is completed. Thus, from a bacterial perspective, phages pose a persistent lethal threat to bacterial populations.
Giedrius Gasiunas +2 more
core +1 more source
Pressure-dependent 13C chemical shifts in proteins: Origins and applications [PDF]
, 2009 Pressure-dependent (13)C chemical shifts have been measured for aliphatic carbons in barnase and Protein G. Up to 200 MPa (2 kbar), most shift changes are linear, demonstrating pressure-independent compressibilities.
Akasaka, A. +3 more
core +1 more source
An improved CRISPRi system in Pichia pastoris
Synthetic and Systems Biotechnology, 2023 CRISPR interference (CRISPRi) has been developed and widely used for gene repression in various hosts. Here we report an improved CRISPRi system in Pichia pastoris by fusing dCas9 with endogenous transcriptional repressor domains.
Shujing Qiao +4 more
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