Inhibition of HIV-1 Viral Infection by an Engineered CRISPR Csy4 RNA Endoribonuclease. [PDF]
PLoS ONE, 2015 The bacterial defense system CRISPR (clustered regularly interspaced short palindromic repeats) has been explored as a powerful tool to edit genomic elements.
Rui Guo +5 more
doaj +1 more source
The inhibition of IRE1alpha/XBP1 axis prevents EBV-driven lymphomagenesis in NSG mice
Microbiology Spectrum, 2023 Post-transplant lymphoproliferative disorders (PTLD) are a group of heterogeneous diseases originating in conditions of immune deficiency, whose main driver is considered to be Epstein-Barr virus (EBV).
Andrea Arena +9 more
doaj +1 more source
The endoplasmic reticulum in plant immunity and cell death [PDF]
, 2012 The endoplasmic reticulum (ER) is a highly dynamic organelle in eukaryotic cells and a major production site of proteins destined for vacuoles, the plasma membrane, or apoplast in plants.
Eichmann, Ruth, Schäfer, P. (Patrick)
core +2 more sources
Pumilio protects Xbp1 mRNA from regulated Ire1-dependent decay
Nature Communications, 2022 In Drosophila, ER-targeted mRNAs are degraded by Ire1-dependent pathway. Here the authors report that the fly mRNA binding protein Pumilio is phosphorylated by Ire1 and binds to Xbp1 mRNA, protecting it from the non-canonical endoribonuclease activity of
Fátima Cairrão +5 more
doaj +1 more source
Drosophila CG3303 is an essential endoribonuclease linked to TDP-43-mediated neurodegeneration [PDF]
, 2017 Endoribonucleases participate in almost every step of eukaryotic RNA metabolism, acting either as degradative or biosynthetic enzymes. We previously identified the founding member of the Eukaryotic EndoU ribonuclease family, whose components display ...
BOZZONI, Irene +11 more
core +1 more source
Novel specificity of an endoribonuclease of yeast [PDF]
FEBS Letters, 1986 Investigations of the cleavage specificity of a cyclizing endoribonuclease of yeast show that it hydrolyzes Y‐A bonds of single‐stranded regions of RNA and that oligonucleotides are hydrolyzed poorly. The stringent specificity of the enzyme suggests that it may be useful for generating specific fragments of polyribonucleotides.
openaire +3 more sources
RNA Interference by Single- and Double-stranded siRNA With a DNA Extension Containing a 3′ Nuclease-resistant Mini-hairpin Structure [PDF]
, 2013 Selective gene silencing by RNA interference (RNAi) involves double-stranded small interfering RNA (ds siRNA) composed of single-stranded (ss) guide and passenger RNAs.
Ahmed +38 more
core +6 more sources
Mechanistic understanding of human SLFN11
Nature Communications, 2022 Schlafen 11 serves as an antiviral restriction factor and a predictive biomarker in cancer. Here, the authors use cryoelectron microscopy and biochemical assays to understand tRNA endoribonuclease and DNA binding functions of human Schlafen 11.
Felix J. Metzner +5 more
doaj +1 more source
The structure of Rph, an exoribonuclease from Bacillus anthracis, at 1.7 angstrom resolution [PDF]
, 2008 Maturation of tRNA precursors into functional tRNA molecules requires trimming of the primary transcript at both the 5' and 3' ends. Cleavage of nucleotides from the 3' stem of tRNA precursors, releasing nucleotide diphosphates, is accomplished in ...
Blagova, Elena V. +5 more
core +2 more sources
Inhibition of IRE1α-mediated XBP1 mRNA cleavage by XBP1 reveals a novel regulatory process during the unfolded protein response [PDF]
, 2017 Background: The mammalian endoplasmic reticulum (ER) continuously adapts to the cellular secretory load by the activation of an unfolded protein response (UPR).
Bulleid, Neil J. +4 more
core +2 more sources