Results 131 to 140 of about 5,282 (158)

Bacteriophage T4 RegB Endoribonuclease

2001
Publisher Summary The development of bacteriophage T4 is largely determined by a precisely coordinated subversion of the host RNA polymerase to permit initiation from different classes of T4-specific promoters. The synthesis, in cascade, of regulatory elements that modify the transcription apparatus, ensures the temporal ordering of the developmental
openaire   +2 more sources

The Cmr complex: an RNA-guided endoribonuclease

Biochemical Society Transactions, 2013
The CRISPR (clustered regularly interspaced short palindromic repeats)–Cas (CRISPR-associated) system protects prokaryotes from infection by viruses and other potential genome invaders. This system represents an inheritable and adaptable immune system that is mediated by large ribonucleoprotein complexes, the CRISPR–Cas effector complexes.
openaire   +2 more sources

Endoribonuclease from bovine adrenal cortex cytosol

Biochemistry, 1975
An endoribonuclease which digests a variety of synthetic homoribopolymers and poly(A)-rich mRNA has been identified and purified greater than 500-fold with respect to specific activity from bovine adrenal cortex cytosol. Enzymatic digestion of synthetic poly(riboadenylic acid) was stimulated by Mn-2+ and Mg-2+ and the enzyme exhibited broad pH and salt
H I, Miller, L A, Perkins, M G, Rosenfeld   +2 more
openaire   +2 more sources

Endoribonuclease activity associated with animal RNA viruses

Journal of Virology, 1978
A specific endoribonucleolytic activity was detected when detergent-lysed vesicular stomatitis of Sendai virus was incubated with the precursor to Escherichia coli tRNA Tyr. The cleavage products produced and the characteristics of the reaction were similar to those previously reported for human KB cell RNase NU.
D, Kolakofsky, S, Altman
openaire   +2 more sources

Endoribonuclease IV. 2. Further Investigation on the Specificity

European Journal of Biochemistry, 1976
The poly(A)-specific endoribonuclease IV produces oligo(A) fragments of a chain length of 10 AMP nucleotides. One enzyme molecule performs 1700 cleavages per min; the cleavages occur randomly. The endoribonuclease IV is a nuclear enzyme which is present in the oviduct of quails in a concentration of 40 000 enzyme molecules per cell. Poly(A) segments on
W E, Müller, G, Seibert, R, Steffen, R K, Zahn   +3 more
openaire   +2 more sources

Cleavage site recognition by the tRNA splicing endoribonuclease

Gene, 1993
A single tRNA-splicing endoribonuclease can cleave several precursors. In addition to the conserved nucleotides (nt), there are positions in the mature domain that, though not always occupied by the same nt, nevertheless play a fundamental role in intron excision reaction. The elements of the recognition set (invariant nt, nt at the cardinal positions)
G P, Tocchini-Valentini, M I, Baldi, D, Gandini-Attardi, E, Mattoccia   +3 more
openaire   +2 more sources

RNA Recognition and Cleavage by Sequence-Specific Endoribonucleases

Protein & Peptide Letters, 2007
Inactivation of RNA molecules by sequence-specific endoribonucleolytic cleavage is a subtle mechanism by which cells regulate gene expression. Sequence-specific endoribonucleases can recognize and cleave particular phosphodiester bonds confined within hundreds/thousands of chemically similar bonds.
Fakhri, Saïda, Benoît, Odaert
openaire   +2 more sources

[Synthetic endoribonucleases].

Molekuliarnaia biologiia, 1993
The data on the synthesis of RNA-fragments with endoribonuclease activity were reviewed. In the framework of the hammerhead model refined by Haselof and Gerlach the contribution of certain nucleotides or functional groups in ribozyme catalytic activity and the role of double helices are considered in detail.
openaire   +1 more source

Designing Efficient and Specific Endoribonuclease-Prepared siRNAs

2012
RNA interference (RNAi) has grown to be one of the main techniques for loss-of-function studies, leading to the elucidation of biological function of genes in various cellular systems and model organisms. While for many invertebrates such as Drosophila melanogaster (D. melanogaster) and Caenorhabditis elegans (C.
Surendranath V, Theis M, Habermann BH, Buchholz F.   +3 more
openaire   +3 more sources

Structure and function of Zucchini endoribonuclease in piRNA biogenesis

Nature, 2012
PIWI-interacting RNAs (piRNAs) silence transposons to maintain genome integrity in animal germ lines. piRNAs are classified as primary and secondary piRNAs, depending on their biogenesis machinery. Primary piRNAs are processed from long non-coding RNA precursors transcribed from piRNA clusters in the genome through the primary processing pathway ...
Hiroshi, Nishimasu, Hirotsugu, Ishizu, Kuniaki, Saito, Satoshi, Fukuhara, Miharu K, Kamatani, Luc, Bonnefond, Naoki, Matsumoto, Tomohiro, Nishizawa, Keita, Nakanaga, Junken, Aoki, Ryuichiro, Ishitani, Haruhiko, Siomi, Mikiko C, Siomi, Osamu, Nureki   +13 more
openaire   +2 more sources