Results 1 to 10 of about 6,256,288 (301)

Multi-locus CRISPRi targeting with a single truncated guide RNA

Nature Communications
A critical goal in functional genomics is evaluating which non-coding elements contribute to gene expression, cellular function, and disease. Functional characterization remains a challenge due to the abundance and complexity of candidate elements. Here,
Molly M. Moore, Siddarth Wekhande, Robbyn Issner, Alejandro Collins, Anna J. Cruz, Yanjing V. Liu, Nauman Javed, Salvador Casaní-Galdón, Jason D. Buenrostro, Charles B. Epstein, Eugenio Mattei, John G. Doench, Bradley E. Bernstein, Noam Shoresh, Fadi J. Najm   +14 more
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Structural basis underlying viral hijacking of a histone chaperone complex

Nature Communications, 2016
The Epstein-Barr virus tegument protein BNRF1 is required for the establishment of selective viral gene expression during latency and interacts with the histone chaperone DAXX.
Hongda Huang, Zhong Deng, Olga Vladimirova, Andreas Wiedmer, Fang Lu, Paul M. Lieberman, Dinshaw J. Patel   +6 more
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TENT2, TUT4, and TUT7 selectively regulate miRNA sequence and abundance

Nature Communications, 2022
The authors reveal insights into miRNA tailing specificity of TENTs and their effect on miRNA levels by combinatory KOs of TENT2, TUT4 and TUT7. They show uridylation is linked to TENT2 and identify non-redundant functions between TUT4 and TUT7.
Acong Yang, Xavier Bofill-De Ros, Ryan Stanton, Tie-Juan Shao, Patricia Villanueva, Shuo Gu   +5 more
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Invasion Precedes Tumor Mass Formation in a Malignant Brain Tumor Model of Genetically Modified Neural Stem Cells

Neoplasia: An International Journal for Oncology Research, 2011
Invasiveness, cellular atypia, and proliferation are hallmarks of malignant gliomas. To effectively target each of these characteristics, it is important to understand their sequence during tumorigenesis. However, because most gliomas are diagnosed at an
Oltea Sampetrean, Isako Saga, Masaya Nakanishi, Eiji Sugihara, Raita Fukaya, Nobuyuki Onishi, Satoru Osuka, Masaki Akahata, Kazuharu Kai, Hachiro Sugimoto, Atsushi Hirao, Hideyuki Saya   +11 more
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AGO-bound mature miRNAs are oligouridylated by TUTs and subsequently degraded by DIS3L2

Nature Communications, 2020
3′ end of microRNAs binds to the PAZ domain of Argonaute (AGO) proteins. Here the authors show that terminal nucleotidyltransferases TUT4/7 and exonuclease DIS3L2 induce tailing and decay of 3’ end exposed-microRNAs in AGO PAZ mutant expressing- or ...
Acong Yang, Tie-Juan Shao, Xavier Bofill-De Ros, Chuanjiang Lian, Patricia Villanueva, Lisheng Dai, Shuo Gu   +6 more
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The Penn State Protein Ladder system for inexpensive protein molecular weight markers

Scientific Reports, 2021
We have created the Penn State Protein Ladder system to produce protein molecular weight markers easily and inexpensively (less than a penny a lane). The system includes plasmids which express 10, 15, 20, 30, 40, 50, 60, 80 and 100 kD proteins in E. coli.
Ryan T. Santilli, John E. Williamson, Yoshitaka Shibata, Rosalie P. Sowers, Andrew N. Fleischman, Song Tan   +5 more
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Mapping functional non-coding variation in individual human genomes through haplotyping, multiomics, and deep learning

Nature Communications
Most genetic variants in the human genome reside in non-coding regions, where they can perturb regulatory element activity to influence gene expression, thereby contributing to various phenotypes and diseases.
Mikhail D. Magnitov, Robin H. van der Weide, Aster F. Witvliet, Miguel Hernández-Quiles, Moreno Martinović, Hans Teunissen, Luca Braccioli, Michiel Vermeulen, Elzo de Wit   +8 more
doaj   +1 more source

Chromatin protein complexes involved in gene repression in lamina-associated domains

The EMBO Journal
Lamina-associated domains (LADs) are large chromatin regions that are associated with the nuclear lamina (NL) and form a repressive environment for transcription. The molecular players that mediate gene repression in LADs are currently unknown.
Stefano G Manzo, Abdelghani Mazouzi, Christ Leemans, Tom van Schaik, Nadia Neyazi, Marjon S van Ruiten, Benjamin D Rowland, Thijn R Brummelkamp, Bas van Steensel   +8 more
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Comparative genetic, proteomic and phosphoproteomic analysis of C. elegans embryos with a focus on ham-1/STOX and pig-1/MELK in dopaminergic neuron development

Scientific Reports, 2017
Asymmetric cell divisions are required for cellular diversity and defects can lead to altered daughter cell fates and numbers. In a genetic screen for C.
Sarah-Lena Offenburger, Dalila Bensaddek, Alejandro Brenes Murillo, Angus I. Lamond, Anton Gartner   +4 more
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LncRNA SNHG17 interacts with LRPPRC to stabilize c-Myc protein and promote G1/S transition and cell proliferation

Cell Death and Disease, 2021
Oncogenic c-Myc is a master regulator of G1/S transition. Long non-coding RNAs (lncRNAs) emerge as new regulators of various cell activities. Here, we found that lncRNA SnoRNA Host Gene 17 (SNHG17) was elevated at the early G1-phase of cell cycle.
Jin-Yu Liu, Ya-Jing Chen, Huan-Hui Feng, Zhan-Li Chen, Yun-Long Wang, Jin-E Yang, Shi-Mei Zhuang   +6 more
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