Results 171 to 180 of about 5,849 (189)
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Nucleotide binding architecture for secreted cytotoxic endoribonucleases
Biochimie, 2013Vertebrate secreted RNases are small cationic protein endowed with an endoribonuclease activity that belong to the RNase A superfamily and display diverse cytotoxic activities. In an effort to unravel their mechanism of action, we have analysed their nucleotide binding recognition patterns. General shared features with other nucleotide binding proteins
Ester, Boix +3 more
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Designing Efficient and Specific Endoribonuclease-Prepared siRNAs
2012RNA interference (RNAi) has grown to be one of the main techniques for loss-of-function studies, leading to the elucidation of biological function of genes in various cellular systems and model organisms. While for many invertebrates such as Drosophila melanogaster (D. melanogaster) and Caenorhabditis elegans (C.
Surendranath V +3 more
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Endoribonucleases – enzymes gaining spotlight in mRNA metabolism
The FEBS Journal, 2010The efficient turnover of messenger RNA represents an important mechanism that allows the cell to control gene expression. Until recently, the mechanism of mRNA decay was mainly attributed to exonucleases, comprising enzymes that degrade RNAs from the ends of the molecules.
Wai Ming, Li +2 more
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Bacteriophage T4 RegB Endoribonuclease
2001Publisher Summary The development of bacteriophage T4 is largely determined by a precisely coordinated subversion of the host RNA polymerase to permit initiation from different classes of T4-specific promoters. The synthesis, in cascade, of regulatory elements that modify the transcription apparatus, ensures the temporal ordering of the developmental
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A calcium dependent endoribonuclease from Trypanosoma congolense cytoplasm
Molecular and Biochemical Parasitology, 1984A major endoribonuclease whose activity depends on the presence of calcium ions was isolated from the cytoplasm of Trypanosoma congolense. Like the Ca2+-dependent endoribonuclease of T. brucei cytoplasm, this enzyme degraded poly(A) preferentially but also degraded to a lesser extent poly(U), tRNA and rRNA to give 5'-phosphoribosyl oligonucleotides ...
G O, Gbenle, E O, Akinrimisi
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Purification and characterization of a novel mammalian endoribonuclease
Journal of Cellular Biochemistry, 2005AbstractEndonuclease‐mediated mRNA decay appears to be a common mode of mRNA degradation in mammalian cells, but yet only a few mRNA endonucleases have been described. Here, we report the existence of a second mammalian endonuclease that is capable of cleaving c‐myc mRNA within the coding region in vitro.
Kirk, Bergstrom +4 more
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Purification of cytosolic, latent endoribonuclease from porcine thyroid
Archives of Biochemistry and Biophysics, 1982Abstract An alkaline endoribonuclease was purified 1800-fold from the cytosolic, latent ribonuclease fraction of porcine thyroids by gentle procedures specifically designed to exclude both heating and acidification steps. Polyacrylamide gel electrophoresis revealed a broad peak of enzyme activity that was coincident with the stained protein band.
E E, Button, R, Guggenheimer, F J, Kull
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The Cmr complex: an RNA-guided endoribonuclease
Biochemical Society Transactions, 2013The CRISPR (clustered regularly interspaced short palindromic repeats)–Cas (CRISPR-associated) system protects prokaryotes from infection by viruses and other potential genome invaders. This system represents an inheritable and adaptable immune system that is mediated by large ribonucleoprotein complexes, the CRISPR–Cas effector complexes.
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Characterization of an Endoribonuclease in Rat-Liver Nucleoli
European Journal of Biochemistry, 1974Synthetic RNAs, i.e. [3H]poly(U) and [14C]poly(A), and natural RNAs, i.e. 16-S and 23-S Escherichia coli ribosomal [14C]RNA and MS2 coliphage [3H]RNA, were incompletely hydrolyzed by a rat liver nucleolar enzyme as measured by solubilization of radioactivity following ethanol precipitation.
A, Boctor, A, Grossman, W, Szer
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Cleavage site recognition by the tRNA splicing endoribonuclease
Gene, 1993A single tRNA-splicing endoribonuclease can cleave several precursors. In addition to the conserved nucleotides (nt), there are positions in the mature domain that, though not always occupied by the same nt, nevertheless play a fundamental role in intron excision reaction. The elements of the recognition set (invariant nt, nt at the cardinal positions)
G P, Tocchini-Valentini +3 more
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