Results 71 to 80 of about 162,209 (266)

Investigation of seasonal soil moisture and temperature variations underneath a waffle raft foundation built on reactive soil

Scientific Reports
Lightweight structures, such as residential foundations, built on reactive soils experience stability issues due to the soil’s high shrink-swell movements.
S. Mediliye Gedara, P. L.P. Wasantha, B. Teodosio, E. Yaghoubi, R. van Staden, M. Guerrieri   +5 more
doaj   +1 more source

Challenges for the Ukrainian energy industry

Security and Defence Quarterly, 2014
<i>Reform of the energy sector is very important, both for the Ukrainian economy and for the reputation of Ukraine in Europe. Since independence, the energy sector, and especially the gas sector, has been totally in transparency and a major area of corruption.
openaire   +2 more sources

Energy Efficiency in Industrial Utilities

, 2011
The demand for electrical energy has increased tremendously over the last 25 years; its importance is such that it is now a vital component of any nation’s economic progress. Increase in population has increased the energy requirements, coupled with the industrialization & socio-economic responsibilities; the energy supply has not kept pace with the ...
Kini, P. Giridhar, Bansal, Ramesh C.
openaire   +3 more sources

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids

FEBS Open Bio, EarlyView.
Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão, Diogo M. Engrácia, Graça Soveral, Filipa Mendes   +3 more
wiley   +1 more source

Natural radionuclide occurrence and ingestion dose in foodstuffs from a Uranium Province, Brazil

Scientific Reports
Radiological assessment of dietary intake is a key component of environmental surveillance in areas characterized by enhanced natural radioactivity. In this context, the Lagoa Real Uranium Province, a strategic mineral region in Bahia, Brazil, presents a
Hector Hugo Silva Medrado, Verônica Alves dos Santos, Gisele Gonçalves Costa, Joel Augusto Moura Porto, Rubens Martins Moreira, Carlos Alberto de Carvalho Filho   +5 more
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source

Importin 7 mediates the nuclear import of HIV‐1 integrase via a specific interacting interface

FEBS Open Bio, EarlyView.
HIV‐1 integrase enables viral DNA integration into the host genome. By binding to the core domain of the host protein Importin 7 via its C‐terminal domain, the integrase is transported across the nuclear membrane into the nucleus, where integration of the viral genome into host DNA takes place. This translocation is a critical step for subsequent viral
Juana Bana, Avigail Yariv, Tal Oppenheim, Hadar Amartely, Dana Reichmann, Oded Livnah   +5 more
wiley   +1 more source