Results 231 to 240 of about 1,164,351 (327)

Engineering an Overflow‐Responsive Regulation System for Balancing Cellular Redox and Optimizing Microbial Production

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Escherichia coli accumulates acetate as a byproduct in fast growth aerobic conditions when using glucose as carbon source. This phenomenon, known as overflow metabolism, has negative impacts on cell growth and protein expression, also causes carbon loss during biosynthesis in most microbial production scenarios. In this study, we regarded the “
Jianli Zhang, Jian Wang, Tian Jiang, Xinyu Gong, Qi Gan, Yuxi Teng, Yusong Zou, Ainoor Anwar Dawadi, Yajun Yan   +8 more
wiley   +1 more source

Soft-computing models for predicting plastic viscosity and interface yield stress of fresh concrete. [PDF]

Sci Rep
Inqiad WB, Javed MF, Alsekait DM, Khan NM, Khan M, Aslam F, Elminaam DSA.   +6 more
europepmc   +1 more source

ETV2 Overexpression Promotes Efficient Differentiation of Pluripotent Stem Cells to Endothelial Cells

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Differentiating endothelial cells (ECs) from human pluripotent stem cells (hPSCs) typically takes 2 weeks and requires parameter optimization. Overexpression of cell type‐specific transcription factors in hPSCs has shown efficient differentiation into various cell types.
Yunfeng Ding, Soniya Tamhankar, Feifan Du, Tessa Christopherson, Nate Schlueter, Jenna R. Cohen, Eric V. Shusta, Sean P. Palecek   +7 more
wiley   +1 more source

Global infectious disease surveillance: bridge a 30-metre gap between the International Civil Aviation Organization and the World Anti-Doping Agency. [PDF]

J Glob Health
Huang J, Güllüoğlu M, Döring O, Wang H, Li J, Liu Y.   +5 more
europepmc   +1 more source

Development of an HEK293 Suspension Cell Culture Medium, Transient Transfection Optimization Workflow, and Analytics for Batch rAAV Manufacturing

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Recombinant adeno associated virus (rAAV) vectors have become popular delivery vehicles for in vivo gene therapies, but demand for rAAVs continues to outpace supply. Platform processes for rAAV production are being developed by many manufacturers, and transient chemical transfection of human embryonic kidney 293 (HEK293) cells is currently the
Erica A. Green, Qiang Fu, Nelson Ndhairo, Thomas M. Leibiger, Yongdan Wang, Yongsuk Lee, Kelvin H. Lee, Michael Betenbaugh, Seongkyu Yoon, David J. McNally   +9 more
wiley   +1 more source

Biaxial seismic response of base-column connections in sub-standard steel buildings: dataset. [PDF]

Open Res Eur
Stathas N, Di Sarno L, Wu J, Freddi F, D'Aniello M, Bousias S, Landolfo R, Güneyisi EM, Papagiannis L.   +8 more
europepmc   +1 more source

Small Intestine on a Chip Demonstrates Physiologic Mucus Secretion in the Presence of Lacticaseibacillus rhamnosus Biofilm

Biotechnology and Bioengineering, EarlyView.
Dash and coworkers have fabricated a small intestine on a chip, which encapsulates the physiology and biofilm of the small intestinal epithelium. The dynamic system showed villus differentiation within 5 days of flow, and the mucus layer and biofilm showed small intestinal characteristics.
Sanat Kumar Dash, Cláudia N. H. Marques, Gretchen J. Mahler   +2 more
wiley   +1 more source

Gamifying water crisis management: A serious game for drinking water contamination emergency response. [PDF]

PLoS One
Huang J, Ashcraft CM, Mo W.
europepmc   +1 more source

Analysis of Dynamic Changes in Sedimentation in the Coastal Area of Amir-Abad Port Using High-Resolution Satellite Images. [PDF]

J Imaging
Sam-Khaniani A, Viccione G, Qorbani Fouladi M, Hesabi-Fard R.   +3 more
europepmc   +1 more source

Random Forest Algorithm for the Mechanical Strength Prediction of Green Cement-Based Materials Incorporating Waste Materials Under Fire Condition. [PDF]

Materials (Basel)
Zhang L, Qiu R, Xie J, Liu X, Fu Q, Li Y.   +5 more
europepmc   +1 more source