Results 131 to 140 of about 551,729 (311)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Multi-Strategy Boosted Fick’s Law Algorithm for Engineering Optimization Problems and Parameter Estimation

Biomimetics
To address the shortcomings of the recently proposed Fick’s Law Algorithm, which is prone to local convergence and poor convergence efficiency, we propose a multi-strategy improved Fick’s Law Algorithm (FLAS).
Jialing Yan, Gang Hu, Jiulong Zhang
doaj   +1 more source

Process-parameter-interface model for design management

, 2003
10.1061/(ASCE)0733-9364(2003)129:6(653)Journal of Construction Engineering and Management1296653 ...
Tyagi, A., Bok, S.H., Bok, S. H., Ling, S., Chua, D. K. H., Chua, D.K.H., Ling, San   +6 more
core   +1 more source

Chameleon sequences reveal structural effects in proteins representing micelle‐like distribution of hydrophobicity

FEBS Open Bio, EarlyView.
Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman, Katarzyna Stapor, Leszek Konieczny, Krzysztof Gądek, Piotr Nowakowski   +4 more
wiley   +1 more source

Review of Numerical Simulation of Overburden Grouting in Foundation Improvement

Geotechnics
Overburden layers, composed of unconsolidated sediments, are widely distributed in construction, transportation, and water conservancy projects, but their inherent defects (e.g., developed pores, low strength) easily induce engineering disasters ...
Pengfei Guo, Weiquan Zhao, Linxiu Qu, Xifeng Li, Yahui Ma, Pan Li   +5 more
doaj   +1 more source

Parameter identification and hybrid mathematical modelling techniques applied to non-linear control systems

The study is concerned with the mathematical modelling and parameter identification of nonlinear motion control systems. The control systems under review are the axis motion control systems for a 3-axis CNC machine tool.
Pislaru, Crinela
core  

Performance Evaluation for Maximum Likelihood and Moment Parameter Estimation Methods on Classical Two Weibull Distribution

, 2015
The two-parameter Weibull distribution is the most widely used distribution in reliability engineering. Parameter estimation is a key issue to apply the Weibull to practical engineering. This paper aims to compare the performance of the maximum parameter
Barabadi, Abbas,, Garmabaki, Amir,, Lu, J.M.,, Fuqing, Yuan,   +3 more
core   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Material parameter estimation and hypothesis testing on a 1D viscoelastic stenosis model: Methodology

, 2013
This is the post-print version of the final published paper that is available from the link below. Copyright @ 2013 Walter de Gruyter GmbH.Non-invasive detection, localization and characterization of an arterial stenosis (a blockage or partial blockage ...
Banks, HT, Birch, MJ, Kruse, C, Brewin, MP, Kenz, ZR, Greenwald, SE, Whiteman, JR, Hu, S, Shaw, S   +8 more
core   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source