Evolution‐guided yeast complementation reveals functional differences in human PSPH variants
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez +6 more
wiley +1 more source
Recent advances in Ti<sub>3</sub>C<sub>2</sub>T <sub><i>x</i></sub> MXenes for photonic applications. [PDF]
Trinh Phan TT +3 more
europepmc +1 more source
In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton +6 more
wiley +1 more source
Solution-based synthesis of a CZTS/rGO nanocomposite: structural, electrical and optical studies for solar cell applications. [PDF]
Nayak P +4 more
europepmc +1 more source
Adenosine triphosphate as a modulator of protein interactions and stability
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley +1 more source
Enhanced spin-to-charge conversion in epitaxial La<sub>0.67</sub>Sr<sub>0.33</sub>MnO<sub>3</sub>/NdNiO<sub>3</sub> bilayers at the nickelate metal-insulator phase transition. [PDF]
Sahoo B +13 more
europepmc +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Permanent Oxidative Dipole Engineering in Quinoline COFs for Boosting Exciton Dissociation and Oxygen Activation in H<sub>2</sub>O<sub>2</sub> Photosynthesis. [PDF]
Wu YQ, Zuo ZB, Cui WR.
europepmc +1 more source
Heterotropic regulation and negative homotropic cooperativity
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea +5 more
wiley +1 more source
Enhancing the Source-Side Transient Response of Logarithmic Double-Spiral Terahertz Photoconductive Antennas Using a Central-Feed-Boundary Tip Array. [PDF]
Xu C +5 more
europepmc +1 more source

