Results 51 to 60 of about 5,777 (293)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

Case Study of the Environmental Noise and its Perception in the City of Cluj-Napoca, Romania

open access: yesArchives of Acoustics, 2020
The noise perceiving issue is very subjective and depends on several factors, such as: the living environment in which each person has grown and developed, the education they have received, the culture in which their life principles have formed and, last
Diana Ioana POPESCU
doaj   +1 more source

Impact of road traffic noise on annoyance and preventable mortality in European cities: A health impact assessment

open access: yesEnvironment International, 2022
Background: Road traffic is the main source of environmental noise in European cities and one of the main environmental risks to health and wellbeing. In this study we aimed to provide an in-depth assessment of available road traffic noise data and to ...
Sasha Khomenko   +12 more
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

open access: yesFEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova   +2 more
wiley   +1 more source

People exposed to traffic noise in european agglomerations from noise maps. A critical review

open access: yesNoise Mapping, 2014
Two of the main objectives of the European Directive on environmental noise are, firstly, to unify acoustic indices for assessing environmental noise and, secondly, to standardize assessment methodologies.
Arana Miguel   +2 more
doaj   +1 more source

Noise assessment of the small-scale wind farm [PDF]

open access: yesE3S Web of Conferences, 2019
The importance of renewable energy and especially of eolian systems is growing. For this reason, we propose the investigation of an important pollutant - the noise, which has become so important that European Commission and European Parliament introduced
Alionte Cristian-Gabriel   +1 more
doaj   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

open access: yesFEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya   +2 more
wiley   +1 more source

Burden of disease due to transportation noise in the Nordic countries

open access: yes, 2023
Environmental noise is of considerable public health concern, and quantification of the health impacts is important for preventive strategies. In this project we estimated the burden of disease due to road traffic and railway noise in four Nordic ...
Selander, J,   +9 more
core   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

open access: yesFEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas   +6 more
wiley   +1 more source

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