Results 211 to 220 of about 24,035,647 (351)

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

A Simple and Visual Colorimetric Aptasensor Based on AuNPs for the Rapid Detection of Sulfamethazine in Environmental Samples. [PDF]

Biosensors (Basel)
Chai L, Wang Y, Jiang S, Wang X, Xie Y, Le T.   +5 more
europepmc   +1 more source

Lost, but Found with Nile Red: A Novel Method for Detecting and Quantifying Small Microplastics (1 mm to 20 μm) in Environmental Samples.

Environmental Science and Technology, 2017
Gabriel Erni-Cassola, Matthew I. Gibson, Richard C. Thompson, J. Christie-Oleza   +3 more
semanticscholar   +1 more source

In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols

FEBS Open Bio, EarlyView.
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton, Fang Zhao, Reeta Davis, Meg Walsh, Kevin E O'Connor, David O'Connell, Tanja Narancic   +6 more
wiley   +1 more source

A colorimetric method for detecting virulent bacteriophage to Vibrio cholerae in fecal and environmental samples. [PDF]

PLoS Negl Trop Dis
Luo W, Dinowitz D, Camilli A, Andrews JR, da Silva KE, Debes AK, Sayeed MA, Nelson EJ, Sack DA.   +8 more
europepmc   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

The hidden fluorine: a critical review of analytical methods for unmasking PFAS and closing fluorine mass balance in environmental samples. [PDF]

Anal Bioanal Chem
Balasuriya D, Aguilar JMN, Hoepker AC, Wallace JS, Aga DS.   +4 more
europepmc   +1 more source

Real-Time PCR Method for Detection of Salmonella spp. in Environmental Samples

Applied and Environmental Microbiology, 2017
K. Kasturi, T. Drgon
semanticscholar   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Application of Isotachophoresis to Differentiate Organic Matter in Environmental Samples. [PDF]

Molecules
Kosobucki P.
europepmc   +1 more source