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D-Penicillamine and Enzymatic Activities

Enzyme, 2017
The influence of incubation with D-penicillamine on pure enzyme preparations and on enzymatic activities of serum and skin homogenates was investigated. Three of the nine enzymatic activities studied underwent significant changes. Such effects of D-penicillamine must be taken into consideration if therapeutic or unwanted actions of this drug are to be ...
W, Raab, B, Gmeiner, P, Muckenhuber
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HPLC assay of enzymatic activities

Chromatographia, 1987
Instead of traditional methods, high-performance liquid chromatography can be used for the determination of enzymatic activities. Assays can be performed once the reaction mixture have been injected in the HPLC apparatus. The elution of the samples is carried out with suitable buffers, and the product(s) formed and left oven substrate(s) are ...
PIETTA P, MAURI P, PACE M
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Measuring Enzymatic Activities with Nanopores

ChemBioChem, 2020
AbstractNanopores have become powerful and versatile tools for measuring single molecules since their emergence in the mid‐1990s. They can be used to sense a wide variety of analytes including metal ions, small organic molecules, DNA/RNA, proteins, etc. to monitor chemical reactions, and to sequence DNA.
Yingying Sheng   +3 more
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Pancreatin with High Enzymatic Activity*, †

Journal of the American Pharmaceutical Association (Scientific ed.), 1951
A simple method of preparing pancreatin with high lipase content is outlined. An analysis, by 50 independent workers, of the pancreatin prepared by this method shows that it contains also over three times the amount of trypsin and of amylase required by the U. S. P. Thus it is an easily prepared “triple strength” pancreatin of high lipase content.
C W, BAUER, A J, VAZAKAS
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Enzymatic activity of aphroproteins.

Biochemistry. Biokhimiia, 1999
Chitinase and proteinase activities were found in aphroproteins excreted by larvae of the cicada Aphrophora costalis Mats; this accounts for their fungicidal effect. Aphroproteins did not show DNase or RNase activities and did not exhibit properties of proteinase inhibitors.
Maksyutova N.   +5 more
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Enzymatic Activity of Human Carboxylesterases

Current Protocols in Toxicology, 2007
AbstractThe carboxylesterases (CEs) are hydrolytic enzymes that metabolize xenobiotics that contain ester, thioester, or amide bonds. CEs are ubiquitously expressed but are found in highest concentration in membrane‐enriched fractions of the liver. This unit describes assays used to measure the enzymatic activity and tissue distribution of human CEs ...
Matthew K, Ross, Abdolsamad, Borazjani
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Triggering Enzymatic Activity with Force

Nano Letters, 2009
Integrating single molecule force spectroscopy with fluorescence-based techniques allows the manipulation of an enzyme with a periodic stretching and relaxation protocol while simultaneously monitoring its catalytic activity. After releasing the stretching force we observe a higher probability for enzymatic activity at a time of 1.7 s.
Gumpp, H.   +5 more
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Enzymatic activity in the activated-sludge floc matrix

Applied Microbiology and Biotechnology, 1995
The enzymatic activity of activated sludge was investigated with special emphasis on the localization of the enzymes in the sludge floc matrix. Activated sludge from an advanced activated-sludge treatment plant, performing biological N and P removal, was used. An enzymatic fingerprint was established using a panel of six different enzymes.
Frølund, B., Griebe, T., Nielsen, P. H.
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Enzymatic activity in single cells

2019
With the increasing recognition of the importance in addressing cell-to-cell variations for the understanding of complex biological systems, single cell analyses are becoming increasingly important. Presented in this chapter is a highly sensitive approach capable of measuring human topoisomerase 1 (TOP1) activity in single CD133 positive DLD-1 cells ...
Keller, Josephine Geertsen   +2 more
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Microwave Activation of Enzymatic Catalysis

Journal of the American Chemical Society, 2008
Microwave irradiation can be used to regulate biocatalysis. Herein, the utilization of hyperthermophilic enzymes in a microwave reactor is reported. While these enzymes are inactive at low temperatures, they can be activated with microwave irradiation.
Douglas D, Young   +3 more
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