Results 61 to 70 of about 6,659,593 (302)

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

open access: yesFEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi   +5 more
wiley   +1 more source

Enzymatic characterization of the cultivable fungi. +: detectable enzymatic activity. -: non-detectable enzymatic activity.

open access: yes, 2013
Enzymatic characterization of the cultivable fungi. +: detectable enzymatic activity. -: non-detectable enzymatic activity.
Fernando Bolívar-Galiano (490471)   +7 more
core   +1 more source

Astroenzymology – the environmental limits of enzyme activity [PDF]

open access: yes, 2003
Using organisms from extreme terrestrial environments as models for extraterrestrial life may lead us to underestimate the range of environments that life may inhabit.
Daniel, Roy M., Roy Daniel
core   +1 more source

Reactional ultrasonic systems and microwave irradiation for pretreatment of agro-industrial waste to increase enzymatic activity

open access: yesBioresources and Bioprocessing, 2020
Pretreatment of keratinous residues using an ultrasonic reaction system provides greater enzymatic production in less time. This is a promising technology for measuring enzyme activity and microwave processes.
Fabiane Fernanda Czapela   +11 more
doaj   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

open access: yesFEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy   +4 more
wiley   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

open access: yesFEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa   +5 more
wiley   +1 more source

Enzymatic activity assay.

open access: yes, 2018
Enzymatic activities of purified rPDHA and rPDHB measured by reduction of 2,6-DCPIP at OD600. Equimolar mixtures of rPDHA and rPDHB had similar enzymatic activity as the positive control, porcine heart PDHc.
Fanqing Zhang (6065486)   +10 more
core   +1 more source

Stratification of patients with lysosomal acid lipase deficiency by enzyme activity in dried blood spots

open access: yesMolecular Genetics and Metabolism Reports, 2022
Background: Lysosomal acid lipase deficiency (LAL-D) is a phenotypic continuum between the severe Wolman disease and the attenuated cholesteryl ester storage disease (CESD).
Xinying Hong   +3 more
doaj   +1 more source

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

open access: yesFEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source

Enzymatic activity.

open access: yes, 2015
(A) Effect of pH on the enzymatic activity of TpManGH5 at 75°C. (B) Effect of temperature on the enzymatic activity of TpManGH5 at pH 6. The experimental conditions are described in Materials and Methods.
Viviam M. da Silva (543125)   +6 more
core   +1 more source

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