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Improved enzymatic hydrolysis of hair

Forensic Science International, 1993
An enzymatic hair extraction method is proposed for drug analysis. Pronase digestion of various aliquots of hair from a cocaine abuser was preceded by a 2-h incubation with a dithiothreitol solution. The extraction solution was tested to identify possible interferences in the radioimmunoassay and was compared with other hydrolysis methods to assess the
Offidani, C   +2 more
openaire   +3 more sources

Evolution of the Lignin Chemical Structure during the Bioethanol Production Process and Its Inhibition to Enzymatic Hydrolysis

, 2020
To illuminate the lignin evolution after hydrogen peroxide presoaking prior to ammonia fiber expansion pretreatment (H-AFEX) pretreatment and enzymatic hydrolysis, ball-milled wood lignins were sep...
Chao Zhao   +4 more
semanticscholar   +1 more source

Facile fractionation of lignocelluloses by biomass-derived deep eutectic solvent (DES) pretreatment for cellulose enzymatic hydrolysis and lignin valorization

Green Chemistry, 2019
A recyclable and green biomass-derived deep eutectic solvent (DES) pretreatment was developed to deconstruct the recalcitrant structure of Eucalyptus for further cellulose enzymatic saccharification and lignin valorization.
Xiaojun Shen   +6 more
semanticscholar   +1 more source

Acid-Catalyzed Glycerol Pretreatment of Sugarcane Bagasse: Understanding the Properties of Lignin and Its Effects on Enzymatic Hydrolysis

, 2020
In this study, lignin from acid-catalyzed glycerol (AG) pretreatment of sugarcane bagasse was recovered and characterized.
M. Hassanpour   +5 more
semanticscholar   +1 more source

Semicontinuous enzymatic hydrolysis of lignocelluloses

Biotechnology and Bioengineering, 1991
AbstractLignocelluloses (steamed hardwood and hardwood kraft pulp) were semicontinuously hydrolyzed on a large scale [2‐2. 5 kg of substrate vs. 20, 000 IU filter paperase (FPase)] using a 10‐L hydrolysis reactor with an ultrafiltration unit for the recovery and reuse of cellulases.
M, Ishihara   +3 more
openaire   +2 more sources

Mechanisms of enzymatic glycoside hydrolysis

Current Opinion in Structural Biology, 1994
The determination of a large number of three-dimensional structures of glycosidases, both free and in complex with ligands, has provided valuable new insights into glycosidase catalysis, especially when coupled with results from studies of specifically labelled glycosidases and kinetic analyses of point mutants.
J D, McCarter, S G, Withers
openaire   +2 more sources

Enzymatic Hydrolysis of Xylan

Nature, 1953
A PREVIOUS investigation1 has shown that enzymes from Chœtomium globosum are able to hydrolyse wheat straw xylan to xylose with the formation of a series of intermediates, designated a,b,c, … g, which appear on paper-chromatograms with regularly decreasing RF-values, and which are believed to be oligosaccharides with regularly increasing xylose units ...
openaire   +2 more sources

Enzymatic hydrolysis of nucleoside phosphoramidates

Life Sciences, 1973
Abstract The nucleoside phosphoramidate thymidine-5′-phospho-α-naphthylamidate and thymidine-3′-phospho-α-naphthylamidate were prepared as fluorogenic substrates for the study of enzymatic hydrolysis of the PN bond. With these new substrates, the rate and specificity of hydrolysis of the PN bond of the nucleoside phosphoramidate by snake venom and ...
K C, Tsou, K F, Yip
openaire   +2 more sources

Incorporating Lignin into Polyethylene Glycol Enhanced Its Performance for Promoting Enzymatic Hydrolysis of Hardwood

, 2020
Polyethylene glycol (PEG) has been widely applied to improve enzymatic hydrolysis of lignocellulose by reducing enzyme nonproductive binding on lignins.
Chenhuan Lai   +5 more
semanticscholar   +1 more source

Enzymatic hydrolysis of organic phosphorus.

2004
International ...
Quiquampoix, Herve, Mousain, Daniel
openaire   +2 more sources

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