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Enzyme‐Linked Immunosorbent Assays

Current Protocols in Immunology, 1992
AbstractThis unit describes six different ELISA systems for the detection of specific antibodies, soluble antigens, or cell‐surface antigens. In all six systems, soluble reactants are removed from solution after specifically binding to solid‐phase reactants.
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Enzyme-Linked Immunosorbent Assay

Journal of Immunoassay, 2000
(2000). Enzyme-Linked Immunosorbent Assay. Journal of Immunoassay: Vol. 21, No. 2-3, pp. 165-209.
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Development of an enzyme-linked immunosorbent assay for caffeine

Journal of Immunological Methods, 1990
A competitive enzyme-linked immunosorbent assay suitable for the measurement of caffeine in plasma and serum has been developed. Sheep immunised with an immunogen prepared by coupling 7-(5-carboxypentyl)1,3-dimethylxanthine to egg albumin produced antibodies with little crossreactivity with the metabolites of caffeine.
FICKLING, SA   +4 more
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ELISA: Enzyme-Linked Immunosorbent Assay

Hospital Practice, 1978
Similar in design to radioimmunoassay, comparable in sensitivity and specificity but easier, safer, and less expensive, this new diagnostic technique uses enzyme-labeled rather than isotope-labeled reagents. The end point is a color change that can be assessed by colorimetry or with the naked eye.
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Enzyme-Linked Immunosorbant Assay (ELBA)

2003
In general, immunological methods are not very well suited for a quantitative determination of the antigen to be studied. The ELISA technique, however, can be used for a quantitative or at least semiquantitative determination of the concentration of a certain antigen. The method was first introduced by Engvall and Perlmann (1).
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Enzyme-Linked Immunosorbent Assay

1998
Since 1971, enzyme-amplified immunoassays have been developed to enhance the detectability of antigen-antibody reactions. The most commonly applied immunoassay is the enzyme-linked immunosorbent assay (ELISA), in which the antigen-antibody complexes are adsorbed to wells in plastic microtitre plates.
Jeanne Dijkstra, Cees P. de Jager
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Competitive Enzyme-Linked Immunosorbent Assay

2005
Competitive enzyme-linked immunosorbent assay provides an alternative to dual-antibody sandwich enzyme-linked immunosorbent assay and is widely used for the measurement of substances in biological liquids.
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Enzyme-linked immunosorbent assays in murine cryptococcosis

Medical Mycology, 1981
In this investigation, enzyme-linked immunosorbent assay (ElISA) procedures were used to study the time of appearance and the duration of demonstrable antigen and antibody in body fluids of mice with disseminated cryptococcosis. The ELISA antigen procedure detected cryptococcal capsular polysaccharide (CCP) in the serum and urine of infected mice 3 ...
E N, Scott, H G, Muchmore, F G, Felton
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Enzyme-Linked Immunosorbent Assay (ELISA)

2017
Food allergy is a public health concern especially after recognizing its constantly increased prevalence and severity. Despite careful reading of food ingredient statements, food allergic individuals may experience reactions caused by "hidden", "masked", or "contaminated" proteins that are known major allergens.
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Enzyme - Linked Immunosorbent Assay for the Phytotoxin Thevetin

Journal of Immunoassay, 1993
An enzyme-linked immunosorbent assay is reported for monitoring thevetin, an active constituent of the highly poisonous plant Thevetia nerifolia. A thevetin-BSA conjugate was employed as the immunogen and the antibodies raised in rabbits were used for the development of an ELISA. Penicillinase served as the marker enzyme and its conjugation to thevetin
P, Prabhasankar   +4 more
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