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Development of a nanobody-based competitive enzyme-linked immunosorbent assay for the sensitive detection of antibodies against porcine deltacoronavirus. [PDF]
Yu R +13 more
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A highly sensitive enzyme‑linked immunosorbent assay allows accurate measurements of brain‑derived neurotrophic factor levels in human saliva. [PDF]
Akutsu F +4 more
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Detection of staphylococcal enterotoxins A and B in cow milk using antigen capture enzyme-linked immunosorbent assay and dot-blot assays. [PDF]
Purwanasari HN +4 more
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Qualification of an enzyme-linked immunosorbent assay for quantification of anti-Vi IgG in human sera. [PDF]
Carducci M +13 more
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Cascade enzyme-linked immunosorbent assay (CELISA)
Immunoassays are representative biochemical detection methods. Among them, sandwich-type immunoassays, typified by sandwich ELISA, have used in disease diagnosis or biochemical detection with high target selectivity. Horseradish peroxidase and alkaline phosphatase have been typically used for signal amplification in ELISA.
Young-mi, Lee +4 more
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Enzyme-Linked Immunosorbent Assay (ELISA)
2022Enzyme-linked immunosorbent assay (ELISA) is one of the most specific and straightforward assays for detecting biomolecules in research and clinics. With advances in analytical methods, ELISA assay has been constantly optimized to improve its sensitivity, and different types of ELISA are now available to detect various biomarkers. This chapter provides
Mahdis Sadat, Tabatabaei, Marya, Ahmed
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Clickable Enzyme-Linked Immunosorbent Assay
Biomacromolecules, 2011Click chemistry is explored as a potential cost-effective and selective immobilization method for the production of an enzyme-linked immunosorbent assay (ELISA). Coatings were formulated containing either a terminal alkyne or a bicyclo[6.1.0]non-4-yne (BCN) chemical handle, and a diagnostic peptide was subsequently immobilized onto these coatings by ...
Canalle, L.A. +7 more
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Enzyme-Linked Immunosorbent Assay, Elisa
The Journal of Immunology, 1972Abstract A sensitive and simple method for the quantitative determination of antibodies is reported. Tubes coated with antigen are incubated with antiserum followed by an enzyme-labeled preparation of anti-immunoglobulin. The enzyme remaining in the tubes after washing provides a measure of the amount of specific antibodies in the serum.
E. ENGVALL, P. PERLMANN
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