Results 201 to 210 of about 272,083 (260)

Enzyme-Linked Immunosorbent Assay (ELISA)

2022
Enzyme-linked immunosorbent assay (ELISA) is one of the most specific and straightforward assays for detecting biomolecules in research and clinics. With advances in analytical methods, ELISA assay has been constantly optimized to improve its sensitivity, and different types of ELISA are now available to detect various biomarkers. This chapter provides
Mahdis Sadat, Tabatabaei, Marya, Ahmed
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Enzyme-Linked Immunosorbent Assay, Elisa

The Journal of Immunology, 1972
Abstract A sensitive and simple method for the quantitative determination of antibodies is reported. Tubes coated with antigen are incubated with antiserum followed by an enzyme-labeled preparation of anti-immunoglobulin. The enzyme remaining in the tubes after washing provides a measure of the amount of specific antibodies in the serum.
E. ENGVALL, P. PERLMANN
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Enzyme‐Linked Immunosorbent Assays (ELISA)

Current Protocols in Molecular Biology, 1991
AbstractThis unit describes six different ELISA systems for the detection of specific antibodies, soluble antigens, or cell‐surface antigens. In all six systems, soluble reactants are removed from solution after specifically binding to solid‐phase reactants.
P, Hornbeck, S E, Winston, S A, Fuller
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Indirect Competitive Enzyme-Linked Immunosorbent Assay (ELISA)

Cold Spring Harbor Protocols, 2017
The indirect competitive ELISA (indirect cELISA) pits plate-immobilized antigen against antigens in solution for binding to antigen-specific antibody. The antigens in solution are in the test sample and are first incubated with antigen-specific antibody.
Thomas O, Kohl, Carl A, Ascoli
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ELISA: Enzyme-Linked Immunosorbent Assay

Hospital Practice, 1978
Similar in design to radioimmunoassay, comparable in sensitivity and specificity but easier, safer, and less expensive, this new diagnostic technique uses enzyme-labeled rather than isotope-labeled reagents. The end point is a color change that can be assessed by colorimetry or with the naked eye.
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Enzyme-Linked Immunosorbent Assay (ELISA)

2017
Food allergy is a public health concern especially after recognizing its constantly increased prevalence and severity. Despite careful reading of food ingredient statements, food allergic individuals may experience reactions caused by "hidden", "masked", or "contaminated" proteins that are known major allergens.
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Enzyme linked immunosorbent assay (ELISA) for paraquat

International Journal of Immunopharmacology, 1983
An Enzyme Linked Immunosorbent Assay (ELISA) has been developed for the estimation of paraquat. The amount of paraquat present in samples of human plasma was estimated in terms of the degree to which it combined with a rabbit antibody raised to a conjugate to paraquat with bovine serum albumin.
Z, Niewola, S T, Walsh, G E, Davies
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Enzyme-Linked Immunosorbant Assay (ELBA)

2003
In general, immunological methods are not very well suited for a quantitative determination of the antigen to be studied. The ELISA technique, however, can be used for a quantitative or at least semiquantitative determination of the concentration of a certain antigen. The method was first introduced by Engvall and Perlmann (1).
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Automation of enzyme-linked immunosorbent assay (ELISA)

Journal of Immunological Methods, 1979
A prototype of automatized enzyme-linked immunosorbent assay (ELISA) in tubes is described, using a commercially available basic material, easily modified. Nine hundred samples could be completely studied in a day by only one person. The different steps of the automatized ELISA were systematically studied to obtain the best performance. Its application
Carlier, Yves, Bout, D, Capron, André
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