Results 21 to 30 of about 3,915,254 (313)

The alkynyl-containing compounds from mushrooms and their biological activities

open access: yesNatural Products and Bioprospecting, 2023
Mushrooms have been utilized by humans for thousands of years due to their medicinal and nutritional properties. They are a crucial natural source of bioactive secondary metabolites, and recent advancements have led to the isolation of several alkynyl ...
Ji-shuang Qi   +5 more
doaj   +1 more source

Comparative Study of Single-stranded Oligonucleotides Secondary Structure Prediction Tools

open access: yesBMC Bioinformatics, 2023
Background Single-stranded nucleic acids (ssNAs) have important biological roles and a high biotechnological potential linked to their ability to bind to numerous molecular targets. This depends on the different spatial conformations they can assume. The
Thomas Binet   +4 more
doaj   +1 more source

ISFET based enzyme sensors [PDF]

open access: yes, 1987
This paper reviews the results that have been reported on ISFET based enzyme sensors. The most important improvement that results from the application of ISFETs instead of glass membrane electrodes is in the method of fabrication. Problems with regard to
Bergveld, Piet, Schoot, Bart H. van der
core   +2 more sources

Kinetic Characterization and X-ray Structure of a Mutant of Haloalkane Dehalogenase with Higher Catalytic Activity and Modified Substrate Range [PDF]

open access: yes, 1996
Conversion of halogenated aliphatics by haloalkane dehalogenase proceeds via the formation of a covalent alkyl-enzyme intermediate which is subsequently hydrolyzed by water.
Dijkstra, Bauke W.,   +7 more
core   +3 more sources

The length of ribosomal binding site spacer sequence controls the production yield for intracellular and secreted proteins by Bacillus subtilis

open access: yesMicrobial Cell Factories, 2020
Background Bacillus subtilis is widely used for the industrial production of recombinant proteins, mainly due to its high secretion capacity, but higher production yields can be achieved only if bottlenecks are removed.
Kristina Volkenborn   +5 more
doaj   +1 more source

On the quasi-steady state assumption applied to Michaelis-Menten and suicide substrate reactions with diffusion [PDF]

open access: yes, 1991
We consider a recent extension to the validity of the quasi-steady-state assumption (QSSA) which includes the case where the ratio of the initial enzyme to substrate concentration is not necessarily small.
Burke, Meghan   +2 more
core   +1 more source

Clinical Utility of SARS-CoV-2 Antibody Titer Multiplied by Binding Avidity of Receptor-Binding Domain (RBD) in Monitoring Protective Immunity and Clinical Severity

open access: yesViruses, 2023
Conventional serum antibody titer, which expresses antibody level, does not provide antigen binding avidity of the variable region of the antibody, which is essential for the defense response to infection.
Etsuhisa Takahashi   +11 more
doaj   +1 more source

Synergistic action of the gut microbiota in environmental RNA interference in a leaf beetle

open access: yesMicrobiome, 2021
Background RNA interference (RNAi) has emerged as an efficient tool to control insect pests. When insects ingest double-stranded RNAs (dsRNAs) targeted against essential genes, strong gene silencing and mortality can be induced.
Letian Xu   +6 more
doaj   +1 more source

Genotypes diversity of env gene of Bovine leukemia virus in Western Siberia

open access: yesBMC Genetics, 2020
Background This study describes the biodiversity and properties of Bovine leukemia virus in Western Siberia. This paper explores the effect of different genotypes of the env gene of the cattle leukemia virus on hematological parameters of infected ...
Natalia Blazhko   +7 more
doaj   +1 more source

A new intrinsic thermal parameter for enzymes reveals true temperature optima [PDF]

open access: yes, 2004
Two established thermal properties of enzymes are the Arrhenius activation energy and thermal stability. Arising from anomalies found in the variation of enzyme activity with temperature, a comparison has been made of experimental data for the activity ...
Daniel, Roy M.   +4 more
core   +2 more sources

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