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Measurement of Enzyme Activities
2017The determination of enzyme activities in organ or organellar extracts is an important means of investigating metabolic networks and allows testing the success of enzyme-targeted genetic engineering. It also delivers information on intrinsic enzyme parameters such as kinetic properties or impact of effector molecules. This chapter provides protocols on
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Activities of proteolytic enzymes
2011Proteases, also known as proteinases or proteolytic enzymes, are a large group of hydrolases that catalyze the cleavage of peptide bonds in proteins to produce peptides and/or amino acids. Classification of proteolytic enzymes is based on three major criteria: type of reaction catalyzed, functional group of the active site, and type of molecular ...
LANDI, LORETTA +3 more
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Multienzyme Biosensors — Coupled Enzyme Reactions and Enzyme Activation
1993Traditionally enzymes are used as recognition elements to measure substrate molecules by catalyzing the turnover of these species to detectable products. The signal generation at biosensors using immobilized biomolecules is based on the superposition of the following processes: (i) the mass transport of the analyte, (ii) biochemical recognition of the ...
Scheller, Frieder W. +5 more
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Metal–Organic Framework-Based Enzyme Biocomposites
Chemical Reviews, 2021, Peter Wied, Francesco Carraro
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Bioinspired Framework Catalysts: From Enzyme Immobilization to Biomimetic Catalysis
Chemical Reviews, 2023Kun-Yu Wang, Jiandong Pang, Wei Shi
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Expanding the Synthetic Toolbox through Metal–Enzyme Cascade Reactions
Chemical Reviews, 2023Ivan Lavandera, Vicente Gotor-Fernández
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