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Enzymes and Enzyme Activity Encoded by Nonenveloped Viruses
Annual Review of Virology, 2017Viruses are obligate intracellular parasites that rely on host cell machineries for their replication and survival. Although viruses tend to make optimal use of the host cell protein repertoire, they need to encode essential enzymatic or effector functions that may not be available or accessible in the host cellular milieu.
Manidipa Banerjee+2 more
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Multimodulation of Enzyme Activity
1981Publisher Summary This chapter discusses the multimodulation of enzyme activity. Multimodulation of enzyme activity arises from the accumulation in a given enzyme of several regulatory mechanisms, whether of different types—cooperative, allosteric, interconversion, or of the same type—multiple allosteric effects, or of any combination of these ...
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Regulation of enzyme activity in adsorptive enzyme systems
Journal of Theoretical Biology, 1979Abstract The kinetic behaviour of adsorptive enzyme systems with free and adsorbed enzyme forms in rapid equilibrium has been analysed. It has been shown that the dependences of enzymic reaction rate on substrate or “adsorptive effector” concentrations reveal the deviations from simple kinetic laws of Michaelis-Menten type (positive or negative ...
N.I. Loboda, B.I. Kurganov
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Enzymic Activities of Commercial Preparations of Pancreatic Enzymes
Scottish Medical Journal, 1977The enzymic activities of 20 commercial pancreatic extracts have been measured by standard (F.I.P.) methods of assay and the activities have been compared with a standard preparation of pancreatin supplied by the British Pharmacopoeia Commission. A wide range of enzymic activities was found. The tabulated cost per unit of enzymic activities provides a
K. G. Wormsley+3 more
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Measurement of Enzyme Activities
2017The determination of enzyme activities in organ or organellar extracts is an important means of investigating metabolic networks and allows testing the success of enzyme-targeted genetic engineering. It also delivers information on intrinsic enzyme parameters such as kinetic properties or impact of effector molecules. This chapter provides protocols on
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Enzyme Activities in Human Liver
Enzymologia biologica et clinica, 1970SummaryBiologic, technical, and methodic problems in the determination of enzyme activities in human hepatic tissue are evaluated critically and enzyme activities of normal liver, as well as their ...
E. Schmidt, F. W. Schmidt
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Biochemical and Biophysical Research Communications - BBRC, 1994
Hepatocyte growth factor (HGF) is a heterodimer protein, derived from an inactive single-chain precursor (pro-HGF) by the proteolytic cleavage at Arg-Val site.
K. Mizuno+5 more
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Hepatocyte growth factor (HGF) is a heterodimer protein, derived from an inactive single-chain precursor (pro-HGF) by the proteolytic cleavage at Arg-Val site.
K. Mizuno+5 more
semanticscholar +1 more source
Biochemical and Biophysical Research Communications - BBRC, 1998
D-Dopachrome tautomerase converts 2-carboxy-2,3-dihydroindole-5,6-quinone (D-dopachrome) into 5,6-dihydroxyindole. This protein has an amino acid sequence that is highly homologous with that of macrophage migration inhibitory factor (MIF), which has the ...
J. Nishihira+7 more
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D-Dopachrome tautomerase converts 2-carboxy-2,3-dihydroindole-5,6-quinone (D-dopachrome) into 5,6-dihydroxyindole. This protein has an amino acid sequence that is highly homologous with that of macrophage migration inhibitory factor (MIF), which has the ...
J. Nishihira+7 more
semanticscholar +1 more source
Measurement of Enzyme Activity
1982Most people approaching the problem of enzyme purification will be following a standard procedure for measuring enzyme activity. But in some cases, it may be useful to develop an alternative assay, for instance, a rapid spectrophotometric method to avoid queuing for the use of a scintillation counter.
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Measuring the Enzyme Activity of Arabidopsis Deubiquitylating Enzymes
2016Deubiquitylating enzymes, or DUBs, are important regulators of ubiquitin homeostasis and substrate stability, though the molecular mechanisms of most of the DUBs in plants are not yet understood. As different ubiquitin chain types are implicated in different biological pathways, it is important to analyze the enzyme characteristic for studying a DUB ...
Kamila Kalinowska+2 more
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