Results 91 to 100 of about 1,624,448 (258)
Smaller is better: nanobodies meet NMR
FEBS Letters, EarlyView.Nanobodies are single‐domain antigen‐binding fragments derived from camelid heavy chain antibodies. Their small size, high stability, and exceptional specificity make nanobodies uniquely useful probes for NMR studies of protein dynamics, transient conformational states, and protein–protein interactions.
Oleg Y. Dmitriev
wiley +1 more source
, 2011 The aim of this study was to evaluate the diagnostic performance of an enzyme-linked immunospot (ELISPOT) assay for interferon-gamma in patients with suspected genitourinary tuberculosis (TB). A total of 30 patients with suspected genitourinary TB at the
LAI, CHIH-CHENG;TAN, CHE-KIM;LIN, SHENG-HSIANG;LIAO, CHUN-HSIN;HUANG, YU-TSUNG;WANG, CHENG-YI;WANG, JEN-YU;LIN, HEN-I;HSUEH, PO-REN +1 more
core
An enzyme-based formaldehyde assay and its utility in a sponge sterol biosynthetic pathway
, 1998 An enzyme-based assay has been developed and utilized to confirm the production of formaldehyde in the dealkylation of sterol side chains in a marine sponge.
Kathleen Kelly, Russell G. Kerr
core +1 more source
Label‐Free Fluorescent Kinase and Phosphatase Enzyme Assays with Supramolecular Host‐Dye Pairs
ChemistryOpen, 2019 The combination of the macrocyclic hosts p‐sulfonatocalix[4]arene and cucurbit[7]uril with the fluorescent dyes lucigenin and berberine affords two label‐free enzyme assays for the detection of kinase and phosphatase activity by fluorescence monitoring ...
Yan‐Cen Liu +4 more
doaj +1 more source
Inhibition of dipeptidyl peptidase 7 impairs lysophagy in LLOMe‐treated HepG2 cells
FEBS Letters, EarlyView.DPP7 inhibition delays ubiquitin‐dependent lysophagy by attenuating ubiquitin accumulation and the recruitment of SQSTM1 and LC3 to damaged lysosomes, resulting in delayed clearance of damaged lysosomes and impaired restoration of lysosomal homeostasis. Created using FigureLabs (Certificate ID: FL‐PUB‐20260922‐Z99XNX).
Na Yeon Park +8 more
wiley +1 more source
Enzyme activity assay solutions were created according to the table.
, 2013 Enzyme activity assay solutions were created according to the table.
Thomas J. S. Merritt (344991) +2 more
core +1 more source
DNA ligase LigE increases bacterial transformation with damaged extracellular DNA
FEBS Letters, EarlyView.The DNA ligase LigE from the naturally competent Gram‐negative bacterium Neisseria gonorrhoaea increases transformation with cut extracellular DNA. This process is ATP‐dependent and relies on the catalytic activity of LigE; it also requires that LigE contains an intact periplasmic localisation sequence, indicating that ligE functions in the periplasm ...
Jolyn Pan +4 more
wiley +1 more source
Echinococcus multilocularis coproantigen detection by enzyme-linked immunosorbent assay in fox, dog, and cat populations [PDF]
, 1999 A sandwich enzyme-linked immunosorbent assay (ELISA) for the detection of Echinococcus multilocularis coproantigens (EM-ELISA) was developed with polyclonal rabbit (solid phase) and chicken egg (catching) antibodies that were directed against E ...
Deplazes, P. +4 more
core
Time-Dependent Kinetic Complexities in Enzyme Assays: A Review
BiomoleculesIn the present review, the importance of analyzing full progress curves in enzyme assays is discussed. The atypical kinetic behavior that can be potentially displayed by enzymes in the performance of an activity assay, as well as the models explaining ...
Juan Luis Rendón, Juan Pablo Pardo
doaj +1 more source
Cogent Food & Agriculture, 2016 The plant Nymphaea pubescens Willd. (Family: Nymphaeaceae) is edible having medicinal importance. The objective of the study was to analyze the potential hepatoprotective properties of the flowers and pedicels of N.
Jayashree Acharya, Bratati De
doaj +1 more source