Results 81 to 90 of about 1,624,448 (258)

An enzyme-linked molecularly imprinted sorbent assay

, 2000
Based on a molecularly imprinted polymer, a competitive binding assay analogous to competitive enzyme immunoassay has been developed. The assay is specific for the herbicide 2,4-dichlorophenoxyacetic acid and uses, for the first time, an enzyme-labelled ...
Mosbach, Klaus, Dzgoev, Anatoli, Surugiu, Ioana, Haupt, Karsten, Danielsson, Bengt, Ye, Lei, Yilmaz, Ecevit, Dzgoev, Anatoli,, Ye, Lei,, Surugiu, Ioana,, Yilmaz, Ecevit,, Mosbach, Klaus,, Danielsson, Bengt,, Haupt, Karsten,, Lund University., Bengt Danielsson, Ecevit Yilmaz, Ioana Surugiu, Karsten Haupt, Klaus Mosbach, Lei Ye, Anatoli Dzgoev   +21 more
core   +1 more source

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

FEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge, Vivian Pogenberg, Alexander Baumgart, Bente Siebels, Hartmut Schlüter, Michael Hecht‐Bucher, Aymelt Itzen   +6 more
wiley   +1 more source

Study of the Amylolytic Activity of Thermophilic Bacteria Isolated from an Algerian Hot Spring (Azzaba, Skikda)

Biology and Life Sciences Forum
Thermostable amylases are among the most widely used and desirable enzymes in the food industry. Indeed, they guarantee faster reactions at high temperatures, enhanced substrate solubility and reduced microbial contamination and cooling costs.
Sarra Bouaita, Zahra Sayad, Douaa Ziani, Rayane Bouguerba, Mohamed Amine Gomri   +4 more
doaj   +1 more source

Prospecting the protein design landscape

FEBS Letters, EarlyView.
This review outlines the current state of various protein design approaches. We discuss the current possibilities enabled by recently released tools, highlight future avenues to pursue in protein design, and underscore the crucial role of key databases and resources for successful protein design workflows.
Jakob R. Riccabona, Katharina T. Stonig, Jens Meiler, Clara T. Schoeder, Monica L. Fernández‐Quintero   +4 more
wiley   +1 more source

Substitution of antibodies and receptors with molecularly imprinted polymers in enzyme-linked and fluorescent assays [PDF]

, 2001
A new technique for coating microtitre plates with molecularly imprinted polymers (MIP), specific for low-molecular weight analytes (epinephrine, atrazine) and proteins is presented.
Piletska, Elena V., Karim, K., Alessandra Bossi, Lowe, P., Sergey A Piletsky, Turner, Anthony P. F., Bossi, Alessandra, Khalku Karim, Anthony P.F Turner, Piletsky, Sergey A., Elena V Piletska, Philip Lowe, KARIM K., TURNER APF, PILETSKY SA, BOSSI, Alessandra Maria, PILETSKA EV, LOWE P.   +17 more
core   +1 more source

L‐aspartate oxidase provides new insights into fumarate reduction in anaerobic darkness in Synechocystis sp. PCC6803

FEBS Letters, EarlyView.
Synechocystis strains deficient in succinate dehydrogenase (SDH) secrete more succinate than the WT under dark anaerobic conditions, supporting that SDH then primarily acts as SDH, not as a fumarate reductase. L‐aspartate oxidase (Laspo) from Synechocystis is functional under anaerobic conditions, reducing fumarate to succinate.
Kateryna Kukil, Jeffrey A. Hawkes, Cecilia Blikstad, Pia Lindberg   +3 more
wiley   +1 more source

The heterodimeric amino acid transporters (HAT) of the SLC7/SLC3 family: A structure−function relationships and relevance to human pathology

FEBS Letters, EarlyView.
Heterodimeric amino acid transporters consist of SLC7 and SLC3 family proteins arranged in a conserved structural organization. They regulate nutrient transport across cell membranes, supporting essential cellular functions. These transporters also contribute to xenobiotic/drug uptake and distribution.
Mariafrancesca Scalise, Tiziano Mazza, Raffaella Scanga, Lara Console, Michele Galluccio, Cesare Indiveri   +5 more
wiley   +1 more source

The ubiquitin system in normal and infected germinal center B cells

FEBS Letters, EarlyView.
The ubiquitin system plays a central role in germinal center (GC) B cells, influencing differentiation to long‐lived memory B cells. Oncogenic gammaherpesviruses gain access to memory B cells by establishing latency in GC B cells. Mapping ubiquitin mechanisms in normal and infected GC B cells will define specific molecular circuits in B cells germane ...
Destiny Davis, Lesly Mejia, Emily K Moser   +2 more
wiley   +1 more source

Metastatic niche shaped by host factors influences disseminated cancer cell fate

FEBS Letters, EarlyView.
Metastasis is shaped not only by cancer cells but also by the environments they encounter. This review explores how factors such as aging, diet, the microbiome, lifestyle, and environmental exposures remodel organ‐specific niches in the lung, liver, bone, and brain, influencing where metastatic cells survive, remain dormant, or grow, and ultimately ...
Gwennan Delyth Ward, Álvaro Ruiz‐Tabas, Patricia Altea‐Manzano   +2 more
wiley   +1 more source

Adaptation of a Commercial NAD⁺ Quantification Kit to Assay the Base-Exchange Activity and Substrate Preferences of SARM1

Molecules
Here, we report an adapted protocol using the Promega NAD/NADH-Glo™ Assay kit. The assay normally allows quantification of trace amounts of both oxidized and reduced forms of nicotinamide adenine dinucleotide (NAD) by enzymatic cycling, but we now show ...
Ilenia Cirilli, Adolfo Amici, Jonathan Gilley, Michael P. Coleman, Giuseppe Orsomando   +4 more
doaj   +1 more source