Results 61 to 70 of about 1,624,448 (258)

Monoamine oxidase A activity in fibroblasts as a functional confirmation of MAOA variants

JIMD Reports, 2021
Monoamine oxidase A (MAO‐A) deficiency is a rare inborn error of metabolism with impaired degradation of biogenic amines including 5‐hydroxytryptamine (5‐HT), resulting in borderline intellectual disability and behavioral abnormalities.
Tessa M. A. Peters, Irma Lammerts van Bueren, Ben P.B.H. Geurtz, Karlien L. M. Coene, Nicole deLeeuw, Han G. Brunner, Jón J. Jónsson, Michèl A. A. P. Willemsen, Ron A. Wevers, Marcel M. Verbeek   +9 more
doaj   +1 more source

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source

Development of a Simple Assay Method for Adenosine Deaminase via Enzymatic Formation of an Inosine-Tb³⁺ Complex

Sensors, 2019
Adenosine deaminase (ADA), which catalyzes the irreversible deamination of adenosine to inosine, is related to various human diseases such as tuberculous peritonitis and leukemia.
Suji Lee, Heewon Park, Yeongcheol Ki, Hohjai Lee, Min Su Han   +4 more
doaj   +1 more source

Enzyme Immunoassay and Enzyme-Linked Immunosorbent Assay [PDF]

Journal of Investigative Dermatology, 2013
inTrOducTiOn Enzyme immunoassay (EIA) and enzyme-linked immunosorbent assay (ELISA) are both widely used as diagnostic tools in medicine and as quality control measures in various industries; they are also used as analytical tools in biomedical research for the detection and quantification of specific antigens or antibodies in a given sample. These two
Gan, Stephanie D., Patel, Kruti R.
openaire   +2 more sources

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

Development of a polyclonal competitive enzyme-linked immunosorbent assay for detection of antibodies to Ehrlichia ruminantium [PDF]

, 2003
A polyclonal competitive enzyme-linked immunosorbent assay (PC-ELISA) is described for detection of antibodies to Ehrlichia (Cowdria) ruminantium by using a soluble extract of endothelial cell culture-derived E.
Sumption, Keith J, Paxton, Edith, Bell-Sakyi, Lesley   +2 more
core  

Liver organoids: modelling complexity in homeostasis and disease

FEBS Letters, EarlyView.
Studying liver in vitro has been challenging because simple 2D cell cultures fail to capture liver's cellular and architectural complexity. To bridge this gap, scientists increasingly use organoids, 3D liver models which better mimic liver composition and function. This review examines recent advances in liver organoid complexity and realism, discusses
Anna M. Dowbaj, Meritxell Huch
wiley   +1 more source

Molecular and biochemical characterization of carbonic anhydrases of Paracoccidioides

Genetics and Molecular Biology
Carbonic anhydrases (CA) belong to the family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide to bicarbonate. In the present work, we characterized the cDNAs of four Paracoccidioides CAs (CA1, CA2, CA3, and CA4).
Mariana Vieira Tomazett, Fabiana Fonseca Zanoelo, Elisa Flávia Cardoso Bailão, Alexandre Melo Bailão, Clayton Luiz Borges, Célia Maria de Almeida Soares   +5 more
doaj   +1 more source

Autophagy and mitophagy in pancreatic β‐cell homeostasis and their involvement in diabetes pathophysiology

FEBS Letters, EarlyView.
This review focuses on the role of autophagy and mitophagy in maintaining pancreatic β‐cell function and homeostasis. We discuss how genetic defects affecting these pathways contribute to the development of type 1, type 2, monogenic, and gestational diabetes. We further explore their potential as therapeutic targets. Created in BioRender.
Yunkyeong Lee, Mingming Tong, Anna L. Gloyn   +2 more
wiley   +1 more source

Production of antisera against sterigmatocystin and its use in an enzyme-linked immunosorbent assay for sterigmatocystin in barley

, 1986
An enzyme-linked immunosorbent assay for the detection of 3-acetyldeoxynivalenol has been set up with an antiserum preparation raised against a bovine serum albumin - 3-acetyldeoxynivalenol hemiglutarate conjugate.
Kang, A.S., Chan, H.W.S., Morgan, M.R.A.
core   +1 more source