Results 1 to 10 of about 856,218 (156)
Micro- and nanochamber array system for single enzyme assays [PDF]
Scientific Reports, 2023 Arrays of small reaction containers, ranging from 624 femtoliters (10–15 L) to 270 attoliters (10–18 L), for capturing a single enzyme molecule and measuring the activity were developed along with a new reversible sealing system based on a pneumatic ...
Kazuki Iijima +3 more
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Synthesis of Phenylboronic Acid-Functionalized Magnetic Nanoparticles for Sensitive Soil Enzyme Assays [PDF]
Molecules, 2022 Soil enzymes, such as invertase, urease, acidic phosphatase and catalase, play critical roles in soil biochemical reactions and are involved in soil fertility.
Can Li +7 more
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Time-Dependent Kinetic Complexities in Enzyme Assays: A Review [PDF]
BiomoleculesIn the present review, the importance of analyzing full progress curves in enzyme assays is discussed. The atypical kinetic behavior that can be potentially displayed by enzymes in the performance of an activity assay, as well as the models explaining ...
Juan Luis Rendón, Juan Pablo Pardo
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Application of Capillary Electrophoresis with Laser-Induced Fluorescence to Immunoassays and Enzyme Assays [PDF]
Molecules, 2019 Capillary electrophoresis using laser-induced fluorescence detection (CE-LIF) is one of the most sensitive separation tools among electrical separation methods.
Binh Thanh Nguyen, Min-Jung Kang
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Easily Fabricated Microfluidic Devices Using Permanent Marker Inks for Enzyme Assays [PDF]
Micromachines, 2016 In this communication, we describe microfluidic paper analytical devices (μPADs) easily fabricated from commercially available Sharpie ink permanent markers on chromatography paper to colorimetrically detect glucose using glucose oxidase (GOx).
Coreen Gallibu +3 more
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Perspectives in Science, 2014 AbstractThe essential requirements for enzyme assays are described and frequently occurring errors and pitfalls as well as their avoidance are discussed. The main factors, which must be considered for assaying enzymes, are temperature, pH, ionic strength and the proper concentrations of the essential components like substrates and enzymes ...
exaly +2 more sources
Microorganisms, 2021 Biocatalytic transesterification is commonly carried out employing lipases in anhydrous organic solvents since hydrolases usually prefer hydrolysis over acyl transfer in bulk water.
Amanda Staudt +7 more
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New gold pincer-type complexes induce caspase-dependent apoptosis in human cancer cells in vitro [PDF]
Vojnosanitetski Pregled, 2021 Background/Aim. The use of cisplatin as a chemotherapeutic opened the door to the new metal-based drug research. New complexes containing metals such as platinum, palladium, ruthenium and gold have recently been analyzed as potential antitumor agents ...
Zarić Milan M. +9 more
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Molecules, 2022 We examined the ability of two human cytosolic transaminases, aspartate aminotransferase (GOT1) and alanine aminotransferase (GPT), to transform their preferred substrates whilst discriminating against similar metabolites.
Alessio Peracchi, Eugenia Polverini
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