Results 21 to 30 of about 856,317 (255)

A method for assaying deubiquitinating enzymes [PDF]

open access: yesBiological Procedures Online, 1998
A general method for the assay of deubiquitinating enzymes was described in detail using (125)I-labeled ubiquitin-fused alphaNH-MHISPPEPESEEEEEHYC (referred to as Ub-PESTc) as a substrate. Since the tyrosine residue in the PESTc portion of the fusion protein was almost exclusively radioiodinated under a mild labeling condition, such as using IODO-BEADS,
Lee Jae Il   +6 more
openaire   +3 more sources

Nanozyme Applications: A Glimpse of Insight in Food Safety

open access: yesFrontiers in Bioengineering and Biotechnology, 2021
Nanozymes own striking merits, including high enzyme-mimicking activity, good stability, and low cost. Due to the powerful and distinguished functions, nanozymes exhibit widespread applications in the field of biosensing and immunoassay, attracting ...
Long Wu   +6 more
doaj   +1 more source

Enzymes Immobilized into Starch- and Gelatin-Based Hydrogels: Properties and Application in Inhibition Assay

open access: yesMicromachines, 2023
The present work is a review of the research on using hydrogels based on natural biodegradable polymers, starch, and gelatin for enzyme immobilization. This review addresses the main properties of starch and gelatin that make them promising materials in ...
Elena N. Esimbekova   +3 more
doaj   +1 more source

Measurement and subcellular distribution of choloyl-CoA synthetase and bile acid-CoA:amino acid N-acyltransferase activities in rat liver

open access: yesJournal of Lipid Research, 1978
An improved method for assaying choloyl-CoA synthetase activity (E.C. 6.2.1.7) and two methods for specific measurement of bile acid-CoA:amino acid N-acyltransferase activity (E.C. 2.3.1) are described.
P G Killenberg
doaj   +1 more source

Arylaminopropanone Derivatives as Potential Cholinesterase Inhibitors: Synthesis, Docking Study and Biological Evaluation

open access: yesMolecules, 2020
Neurodegenerative diseases in which the decrease of the acetylcholine is observed are growing worldwide. In the present study, a series of new arylaminopropanone derivatives with N-phenylcarbamate moiety (1–16) were prepared as potential ...
Anna Hudcová   +7 more
doaj   +1 more source

Diagnostic and Clinical-Epidemiological Insights into Pompe Disease: A Retrospective Analysis at a Reference Center for Inborn Errors of Metabolism [PDF]

open access: yesJournal of Inborn Errors of Metabolism and Screening
Pompe disease (PD) is characterized by an inborn metabolic error resulting from the deficiency of the enzyme acid alpha-glucosidase, leading to glycogen accumulation within lysosomes. The severity of the disease is related to the age of symptom onset and
Sandra Obikawa Kyosen   +3 more
doaj   +2 more sources

Neonatal Screening for MPS Disorders in Latin America: A Survey of Pilot Initiatives

open access: yesInternational Journal of Neonatal Screening, 2020
Newborn screening enables the diagnosis of treatable disorders at the early stages, and because of its countless benefits, conditions have been continuously added to screening panels, allowing early intervention, aiming for the prevention of irreversible
Francyne Kubaski   +12 more
doaj   +1 more source

On Single-Cell Enzyme Assays in Marine Microbial Ecology and Biogeochemistry

open access: yesFrontiers in Marine Science, 2022
Extracellular enzyme activity is a well-established parameter for evaluating microbial biogeochemical roles in marine ecosystems. The presence and activity of extracellular enzymes in seawater provide insights into the quality and quantity of organic ...
Sachia J. Traving   +15 more
doaj   +1 more source

An in‐gel enzyme assay* [PDF]

open access: yesBiochemistry and Molecular Biology Education, 2004
AbstractTerms to be familiar with before you start to solve the test: signal transduction, growth factor signalling, nerve growth factor (NGF), epidermal growth factor (EGF), mitogen‐activated protein kinases (MAPKs), extracellular signal‐regulated kinases (ERKs), MAPK/ERK kinase (MEK), SDS‐PAGE, [γ‐32P]ATP, autoradiography, caspases, protein kinases ...
openaire   +2 more sources

Uncultured Microbial Phyla Suggest Mechanisms for Multi-Thousand-Year Subsistence in Baltic Sea Sediments

open access: yesmBio, 2019
Energy-starved microbes in deep marine sediments subsist at near-zero growth for thousands of years, yet the mechanisms for their subsistence are unknown because no model strains have been cultivated from most of these groups.
Jordan T. Bird   +11 more
doaj   +1 more source

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