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Clickable Enzyme-Linked Immunosorbent Assay
Biomacromolecules, 2011Click chemistry is explored as a potential cost-effective and selective immobilization method for the production of an enzyme-linked immunosorbent assay (ELISA). Coatings were formulated containing either a terminal alkyne or a bicyclo[6.1.0]non-4-yne (BCN) chemical handle, and a diagnostic peptide was subsequently immobilized onto these coatings by ...
Canalle, L.A. +7 more
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Enzyme-Linked Immunosorbent Assay, Elisa
The Journal of Immunology, 1972Abstract A sensitive and simple method for the quantitative determination of antibodies is reported. Tubes coated with antigen are incubated with antiserum followed by an enzyme-labeled preparation of anti-immunoglobulin. The enzyme remaining in the tubes after washing provides a measure of the amount of specific antibodies in the serum.
E. ENGVALL, P. PERLMANN
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Enzyme‐Linked Immunosorbent Assays
Current Protocols in Immunology, 1992AbstractThis unit describes six different ELISA systems for the detection of specific antibodies, soluble antigens, or cell‐surface antigens. In all six systems, soluble reactants are removed from solution after specifically binding to solid‐phase reactants.
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Enzyme-Linked Immunosorbent Assay
Journal of Immunoassay, 2000(2000). Enzyme-Linked Immunosorbent Assay. Journal of Immunoassay: Vol. 21, No. 2-3, pp. 165-209.
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ELISA: Enzyme-Linked Immunosorbent Assay
Hospital Practice, 1978Similar in design to radioimmunoassay, comparable in sensitivity and specificity but easier, safer, and less expensive, this new diagnostic technique uses enzyme-labeled rather than isotope-labeled reagents. The end point is a color change that can be assessed by colorimetry or with the naked eye.
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Enzyme-Linked Immunosorbant Assay (ELBA)
2003In general, immunological methods are not very well suited for a quantitative determination of the antigen to be studied. The ELISA technique, however, can be used for a quantitative or at least semiquantitative determination of the concentration of a certain antigen. The method was first introduced by Engvall and Perlmann (1).
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Thermometric enzyme linked immunosorbent assay: TELISA
Biochimica et Biophysica Acta (BBA) - Enzymology, 1977A new method, thermometric enzyme linked immunosorbent assay (TELISA), for the assay of endogenous and exogenous compounds in biological fluids is described. It is based on the previously described enzyme linked immunosorbent assay technique, ELISA, but utilizes enzymic heat formation which is measured in an enzyme thermistor unit.
B, Mattiasson +3 more
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Cascade enzyme-linked immunosorbent assay (CELISA)
Biosensors and Bioelectronics, 2009Immunoassays are representative biochemical detection methods. Among them, sandwich-type immunoassays, typified by sandwich ELISA, have used in disease diagnosis or biochemical detection with high target selectivity. Horseradish peroxidase and alkaline phosphatase have been typically used for signal amplification in ELISA.
Young-mi, Lee +4 more
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Enzyme-Linked Immunosorbent Assay
1998Since 1971, enzyme-amplified immunoassays have been developed to enhance the detectability of antigen-antibody reactions. The most commonly applied immunoassay is the enzyme-linked immunosorbent assay (ELISA), in which the antigen-antibody complexes are adsorbed to wells in plastic microtitre plates.
Jeanne Dijkstra, Cees P. de Jager
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Competitive Enzyme-Linked Immunosorbent Assay
2005Competitive enzyme-linked immunosorbent assay provides an alternative to dual-antibody sandwich enzyme-linked immunosorbent assay and is widely used for the measurement of substances in biological liquids.
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