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Enzyme-Linked Immunosorbent Assay (ELISA)
2022Enzyme-linked immunosorbent assay (ELISA) is one of the most specific and straightforward assays for detecting biomolecules in research and clinics. With advances in analytical methods, ELISA assay has been constantly optimized to improve its sensitivity, and different types of ELISA are now available to detect various biomarkers. This chapter provides
Mahdis Sadat, Tabatabaei, Marya, Ahmed
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Clickable Enzyme-Linked Immunosorbent Assay
Biomacromolecules, 2011Click chemistry is explored as a potential cost-effective and selective immobilization method for the production of an enzyme-linked immunosorbent assay (ELISA). Coatings were formulated containing either a terminal alkyne or a bicyclo[6.1.0]non-4-yne (BCN) chemical handle, and a diagnostic peptide was subsequently immobilized onto these coatings by ...
Canalle, L.A. +7 more
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Enzyme‐Linked Immunosorbent Assays
Current Protocols in Immunology, 1992AbstractThis unit describes six different ELISA systems for the detection of specific antibodies, soluble antigens, or cell‐surface antigens. In all six systems, soluble reactants are removed from solution after specifically binding to solid‐phase reactants.
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Enzyme-Linked Immunosorbent Assay, Elisa
The Journal of Immunology, 1972Abstract A sensitive and simple method for the quantitative determination of antibodies is reported. Tubes coated with antigen are incubated with antiserum followed by an enzyme-labeled preparation of anti-immunoglobulin. The enzyme remaining in the tubes after washing provides a measure of the amount of specific antibodies in the serum.
E. ENGVALL, P. PERLMANN
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Enzyme-Linked Immunosorbent Assay
Journal of Immunoassay, 2000(2000). Enzyme-Linked Immunosorbent Assay. Journal of Immunoassay: Vol. 21, No. 2-3, pp. 165-209.
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Development of an enzyme-linked immunosorbent assay for caffeine
Journal of Immunological Methods, 1990A competitive enzyme-linked immunosorbent assay suitable for the measurement of caffeine in plasma and serum has been developed. Sheep immunised with an immunogen prepared by coupling 7-(5-carboxypentyl)1,3-dimethylxanthine to egg albumin produced antibodies with little crossreactivity with the metabolites of caffeine.
FICKLING, SA +4 more
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ELISA: Enzyme-Linked Immunosorbent Assay
Hospital Practice, 1978Similar in design to radioimmunoassay, comparable in sensitivity and specificity but easier, safer, and less expensive, this new diagnostic technique uses enzyme-labeled rather than isotope-labeled reagents. The end point is a color change that can be assessed by colorimetry or with the naked eye.
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Enzyme-Linked Immunosorbant Assay (ELBA)
2003In general, immunological methods are not very well suited for a quantitative determination of the antigen to be studied. The ELISA technique, however, can be used for a quantitative or at least semiquantitative determination of the concentration of a certain antigen. The method was first introduced by Engvall and Perlmann (1).
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Enzyme-Linked Immunosorbent Assays in a Chromatographic Format
Journal of Chromatography A, 1996This paper describes the execution of enzyme-linked immunosorbent assays (ELISA), (i) in immunosorbent columns with antibodies immobilized on porous particles, (ii) where samples and reagents are metered by valves and syringe pumps, (iii) samples, reagents, substrate, and wash buffers are transported into or through the system by high pressure liquid ...
F E Regnier
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