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Enzyme-Linked Immunosorbent Assay
1998Since 1971, enzyme-amplified immunoassays have been developed to enhance the detectability of antigen-antibody reactions. The most commonly applied immunoassay is the enzyme-linked immunosorbent assay (ELISA), in which the antigen-antibody complexes are adsorbed to wells in plastic microtitre plates.
Jeanne Dijkstra, Cees P. de Jager
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Covalently linked peptides for enzyme-linked immunosorbent assay
Journal of Immunological Methods, 1990A general method is described, by which synthetic peptides are covalently linked via their carboxyl group to microtiter plates (CovaLink) for enzyme-linked immunosorbent assay (ELISA). Plates were prepared by this method with an angiotensin II peptide and with an HIV-2 peptide and attachment detected by rabbit anti-angiotensin serum and with a positive
J, Søndergård-Andersen +3 more
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Competitive Enzyme-Linked Immunosorbent Assay
2005Competitive enzyme-linked immunosorbent assay provides an alternative to dual-antibody sandwich enzyme-linked immunosorbent assay and is widely used for the measurement of substances in biological liquids.
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Enzyme-Linked Immunosorbent Assay (ELISA)
2017Food allergy is a public health concern especially after recognizing its constantly increased prevalence and severity. Despite careful reading of food ingredient statements, food allergic individuals may experience reactions caused by "hidden", "masked", or "contaminated" proteins that are known major allergens.
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Enzyme - Linked Immunosorbent Assay for the Phytotoxin Thevetin
Journal of Immunoassay, 1993An enzyme-linked immunosorbent assay is reported for monitoring thevetin, an active constituent of the highly poisonous plant Thevetia nerifolia. A thevetin-BSA conjugate was employed as the immunogen and the antibodies raised in rabbits were used for the development of an ELISA. Penicillinase served as the marker enzyme and its conjugation to thevetin
P, Prabhasankar +4 more
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Enzyme linked immunosorbent assay (ELISA) for paraquat
International Journal of Immunopharmacology, 1983An Enzyme Linked Immunosorbent Assay (ELISA) has been developed for the estimation of paraquat. The amount of paraquat present in samples of human plasma was estimated in terms of the degree to which it combined with a rabbit antibody raised to a conjugate to paraquat with bovine serum albumin.
Z, Niewola, S T, Walsh, G E, Davies
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Enzyme-linked immunosorbent assay for diagnosis of brucellosis
European Journal of Clinical Microbiology, 1987The performance of an ELISA for detection of total antibodies to Brucella spp. was compared with that of the Rose Bengal, standard agglutination and Coombs test in the diagnosis of brucellosis. Sera tested were from 208 patients from whom Brucella melitensis had been isolated, 177 patients with significant results in at least two conventional tests ...
J V, Saz +6 more
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Enzyme-linked immunosorbent assay for detection of arenaviruses
Archives of Virology, 1981Conditions for ELISA by using IgG linked with horseradish peroxidase have been developed for the detection of 6 arenaviruses (Machupo, Junin, Tacaribe, Amapari, LCM and Tamiami). This method allows the detection of arenavirus antigens in various materials; organs and blood in affected animals and infected cell culture fluids.
A P, Ivanov +2 more
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Enzyme-linked immunosorbent assays (ELISAs)
Critical Care Medicine, 2005Yichen, Lai +2 more
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