Results 11 to 20 of about 440,729 (219)

Recombinant expression of Barnase in Escherichia coli and its application in plasmid purification

Microbial Cell Factories, 2021
Background The use of bovine-origin ribonucleases has been part of the standard protocol for plasmid DNA purification. As the field of gene therapy now enters the clinical stage, such enzymes need to be phased out or alternative purification protocols ...
Ram Shankar, Nina Schäffer, Marco Schmeer, Joe Max Risse, Karl Friehs, Martin Schleef   +5 more
doaj   +1 more source

Anticancer Activity of Cytosine Deaminase Enzyme Purified From Local Saccharomyces cerevisia Bread Yeast [PDF]

Journal of Bioscience and Applied Research
The study aimed to extract and purify the enzyme cytosine deaminase from locally manufactured bread yeast Saccharomyces cerevisia. To achieve the goal of the study, bread yeast was made locally and transported to the laboratory for an enzyme extraction ...
Muna Mutter, Zainab Hasan, Milad Mezher
doaj   +1 more source

Isolation and partial Purification and for amylase from diabetic patients and some kinetic properties

Tikrit Journal of Pure Science, 2020
Amylase is an enzyme which is  secreted from  pancreas, and salivary glands (α-Amylase EC3.2.1.1), there is a small amount of this enzyme  presents in the blood ranging between 100–300 I.U/L, when this percentage in the blood would increase,it means ...
Wasan Abd alhassan Hamad, Firas Taher Maher   +1 more
doaj   +1 more source

Optimizing of Protease Purification from Bacillus cereus TD5B by Ammonium Sulfate Precipitation

Chemical Engineering Transactions, 2018
The aim of this research was to optimize the protease purification from Bacillus cereus TD5B by ammonium sulfate precipitation. The material used was protease enzyme from Bacillus cereus TD5B.
Atik Winarti, Nanung A Fitriyanto, Ambar Pertiwiningrum, Zaenal Bachruddin, Yudi Pranoto, Yuny Erwanto   +5 more
doaj   +1 more source

Microbial Enzyme Production Using Lignocellulosic Food Industry Wastes as Feedstock: A Review

Bioengineering, 2016
Enzymes are of great importance in the industry due to their substrate and product specificity, moderate reaction conditions, minimal by-product formation and high yield. They are important ingredients in several products and production processes.
Rajeev Ravindran, Amit K. Jaiswal
doaj   +1 more source

Desenvolvimento de um spot test para o monitoramento da atividade da peroxidase em um procedimento de purificação Development of a spot test for peroxidase activity monitoring during a purification procedure

Química Nova, 2008
In this work we describe both a chromatographic purification procedure and a spot test for the enzyme peroxidase (POD: EC 1.11.1.7). The enzyme was obtained from crude extracts of sweet potatoes and the chromatographic enzyme purification procedure ...
Ana Eliza Zeraik, Fernanda Sant'Ana de Souza, Orlando Fatibello-Filho, Oldair D. Leite   +3 more
doaj   +1 more source

Alcohol Dehydrogenase of a Novel Algae Fermentation Strain Meyerozyma guilliermondii [PDF]

Chemical and Biochemical Engineering Quarterly, 2018
The production of bioethanol from algae has attracted wide attention in the field of energy. A novel algae fermentation strain, Meyerozyma guilliermondii, can produce alginate mlyase and alcohol dehydrogenase (ADH) at the same time.
W. Zhang, Z. Zhang, L. Bao, X. Zhang, H. Cui   +4 more
doaj   +1 more source

Recombinant Production and One-Pot Purification for Enhancing Activity of Haloacid Dehalogenase from Bacillus cereus IndB1

Reaktor, 2021
In recent years we have witnessed the emergence of organohalogen utilization in various chemical-based industries, particularly polymer-based, agricultural, and pharmaceutical sectors.
Enny Ratnaningsih, Sulistiya Nirta Sunaryo, Idris Idris, Rindia Maharani Putri   +3 more
doaj   +1 more source

Isolation, Purification and Characterization of Ethyl Earbamate Degrading Enzyme from Metschnikowia and Its Effect on Wine Aroma

Shipin gongye ke-ji, 2022
In order to study the properties of Ethyl Carbamate (EC) enzyme produced by Metschnikowia, the EC enzyme was separated and purified by ammonium sulfate salting out and ion exchange chromatography, and the enzymatic properties of the purified EC enzyme ...
Fande MENG, Xuewei SHI
doaj   +1 more source

Microbial Fibrinolytic Enzymes as Anti-Thrombotics: Production, Characterisation and Prodigious Biopharmaceutical Applications

Pharmaceutics, 2021
Cardiac disorders such as acute myocardial infarction, embolism and stroke are primarily attributed to excessive fibrin accumulation in the blood vessels, usually consequential in thrombosis.
Chhavi Sharma, Alexander Osmolovskiy, Rajni Singh   +2 more
doaj   +1 more source