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Structural stability of an enzyme biocatalyst
Biochemical Society Transactions, 2007TK (transketolase) undergoes inactivation during biocatalytic processes due to oxidation, substrate and product inhibition, reactivity of aldehyde substrates, irreversible inactivation at low pH, and dissociation of cofactors. However, the contribution of protein denaturation to each of these mechanisms is not fully understood.
P A, Dalby +3 more
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Enzyme Stabilization by Virus-Like Particles
Biochemistry, 2020The properties of enzymes packaged within the coat protein shell of virus-like particles (VLPs) were studied to provide a comprehensive assessment of such factors. Such entrainment did not seem to perturb enzyme function, but it did significantly enhance enzyme stability against several denaturing stimuli such as heat, organic solvents, and chaotropic ...
Soumen Das +3 more
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1979
After an introduction by a chapter on enzyme denaturation by physical chemical, and biological agents, methods for the stabilization of water-soluble enzymes are discussed in this review. this includes screening for intrinsically stabilized enzymes, addition of stabilizing agents such as substrates, solvents, salts or polymers, and chemical ...
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After an introduction by a chapter on enzyme denaturation by physical chemical, and biological agents, methods for the stabilization of water-soluble enzymes are discussed in this review. this includes screening for intrinsically stabilized enzymes, addition of stabilizing agents such as substrates, solvents, salts or polymers, and chemical ...
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The enzyme stability of dehydro-enkephalins
Peptides, 1982Dehydro-enkephalins [delta Ala2]-, [delta Ala3]-, [delta Phe4]-, and [delta Leu5]enkephalins, were examined for their stability to enzymatic hydrolysis by carboxypeptidase Y [EC 3.4.16.1]. The successively liberated amino acids were determined quantitatively by amino acid analyses.
Y, Shimohigashi, H C, Chen, C H, Stammer
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Effect of Salts on Enzyme Stability
Annals of the New York Academy of Sciences, 1988Pour tester la stabilite de trois enzymes en presence de solutions salines, les concentrations croissantes de chlorure de sodium, potassium ou lithium sont ajoutees a la preparation enzymatique. La demi-vie de l'enzyme est determinee. La sepctroscopie Raman permet d'etudier le degre d'organisation de l'environnement aqueux et d'expliquer en partie l ...
D, Combes, W N, Ye, A, Zwick, P, Monsan
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1990
Enzymes are active only when their polypeptide chain is folded into a unique globular conformation with a functional active site. In most naturally occurring enzymes, this globular conformation is only 5 to 10 kcal/mole more stable than unfolded, biologically inactive conformations (1).
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Enzymes are active only when their polypeptide chain is folded into a unique globular conformation with a functional active site. In most naturally occurring enzymes, this globular conformation is only 5 to 10 kcal/mole more stable than unfolded, biologically inactive conformations (1).
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Enzyme stabilization: state of the art
Molecular and Cellular Biochemistry, 1991'Enzyme stabilization' is one of the most important fields in basic and applied enzymology. In basic enzymology, it is of particular relevance to understand enzyme stabilization principles first elucidating how and why the enzymes lose their biological activity and then deriving structure-stability relationships existing in enzymatic molecules.
Gianfreda L, Scarfi' MR
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Additives and Enzyme Stability
Biocatalysis, 1988The polypeptide chain of an enzyme is folded so that the necessary functional groups are brought together in the active site. Conformational changes may disrupt this arrangement and cause loss of enzymic activity. The effect of soluble additives on the unfolding process is discussed.
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Energetics of enzyme stability
Trends in Biotechnology, 2002Abstract Advances in computational protein engineering depend on the development of an accurate treatment of the interactions among the ‘elementary constituents' of enzymes. Most of the current research is focused on finding the right balance in the estimations of energy and entropy.
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Stabilization of Enzymes by Using Thermophiles
2017Manufactured steroid compounds have many applications in the pharmaceutical industry. Due to the chemical complexity and chirality of steroids, there is an increasing demand for enzyme-based bioconversion processes to replace those based on chemical synthesis.
Ana Luisa, Ribeiro +3 more
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