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Additives and Enzyme Stability
Biocatalysis, 1988The polypeptide chain of an enzyme is folded so that the necessary functional groups are brought together in the active site. Conformational changes may disrupt this arrangement and cause loss of enzymic activity. The effect of soluble additives on the unfolding process is discussed.
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Engineering enzymes for stability
Current Opinion in Structural Biology, 1996There have been many recent developments in elaborating the approaches for stabilizing enzymes by stabilizing the folding state, destabilizing the unfolded state and altering the kinetics of unfolding. However, these represent a series of rules of thumb rather than the reliable principles that would be expected of 'engineering'.
A, Shaw, R, Bott
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The enzyme stability of dehydropeptides
Biochemical and Biophysical Research Communications, 1978Abstract A pentapeptide, Z-Gly-Gly-Phe-Phe-Ala · OH (1b) and the corresponding unsaturated pentapeptide, Z-Gly-Gly-Phe-Δ Z Phe-Ala · OH (1a) , have been synthesized. The saturated compound (1b) was rapidly hydrolyzed by both chymotrypsin and thermolysin to the expected products, but the dehydropeptide was completely unhydrolyzed by ...
M L, English, C H, Stammer
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Stabilization of enzymes by their specific antibodies
Enzyme and Microbial Technology, 1991In nature, increased stability of enzymes has often been found to be associated with noncovalent protein-protein interactions. Specific antibodies should be suitable for this purpose. To test this hypothesis, we used a number of model enzymes, complexed them with their specific antibodies, and exposed them and the free enzymes to low and high ...
E Y, Shami +3 more
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Rational engineering of enzyme stability
Journal of Biotechnology, 2004During the past 15 years there has been a continuous flow of reports describing proteins stabilized by the introduction of mutations. These reports span a period from pioneering rational design work on small enzymes such as T4 lysozyme and barnase to protein design, and directed evolution.
Eijsink, V.G. +7 more
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Stabilization of Enzymes by Using Thermophiles
2023Manufactured steroid compounds have many applications in the pharmaceutical industry. Due to the chemical complexity and chirality of steroids, there is an increasing demand for enzyme-based bioconversion processes to replace those based on chemical synthesis.
Ana-Luisa, Ribeiro +4 more
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Structural stability of an enzyme biocatalyst
Biochemical Society Transactions, 2007TK (transketolase) undergoes inactivation during biocatalytic processes due to oxidation, substrate and product inhibition, reactivity of aldehyde substrates, irreversible inactivation at low pH, and dissociation of cofactors. However, the contribution of protein denaturation to each of these mechanisms is not fully understood.
P A, Dalby +3 more
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Nanostructures for enzyme stabilization
Chemical Engineering Science, 2006Recent breakthroughs in nanotechnology have made various nanostructured materials more affordable for a broader range of applications. Although we are still at the beginning of exploring the use of these materials for biocatalysis, various nanostructures have been examined as hosts for enzyme immobilization via approaches including enzyme adsorption ...
Jungbae Kim, Jay W. Grate, Ping Wang
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