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Infection of Culicoides Biting Midges with Epizootic Hemorrhagic Disease Virus
Epizootic hemorrhagic disease virus (EHDV) is transmitted by Culicoides biting midges. Studies aiming to predict the likely spread of EHDV require an understanding of the viral infection and replication kinetics within these insects, including the proportion of the insect population that are able to support virus transmission. Here, we describe methodsChristopher, Sanders, Kerry, Newbrook
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Molecular Serotyping of Epizootic Hemorrhagic Disease Virus
Molecular methods are routinely used for the differential diagnosis and genetic characterization of viral disease of livestock. Real-time, quantitative PCR (qPCR) allows RNA/DNA sequence detection and quantification and is considered the gold standard diagnostic method for most viruses.openaire +2 more sources
Whole Genome Sequencing of Epizootic Hemorrhagic Disease Virus
Next-generation sequencing (NGS) technologies are continuously being developed and are becoming a more cost-effective tool for the characterization of viral genomes. Whole genome sequencing of segmented viruses, such as epizootic hemorrhagic disease virus (EHDV), provides insights into the molecular epidemiology as well as such viral evolutionary ...openaire +2 more sources
TRANSMISSION OF TWO STRAINS OF EPIZOOTIC HEMORRHAGIC DISEASE VIRUS IN DEER BY Culicoides variipennis
Journal of Wildlife Diseases, 1977Two strains of epizootic hemorrhagic disease virus (EHDV), New Jersey (NJ) and Kentucky (KY), of deer were biologically transmitted between white-tailed deer, Odocoileus virginianus, by Culicoides variipennis. The KY strain, isolated from C. variipennis collected during an epizootic in deer, was identified as EHDV by serological tests.
N. M. FOSTER +4 more
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Virus Isolation of Epizootic Hemorrhagic Disease Virus in Cell Culture
Virus isolation is used to assist in the diagnosis and confirmation of viral infections. Successful isolation of a virus is highly dependent upon the quality of starting material. Here we describe the preparation and isolation of epizootic hemorrhagic disease virus (EHDV) from blood and tissue samples in tissue culture flasks (TCFs) through the ...openaire +2 more sources
Real-Time RT-PCR Assays for Typing of Epizootic Hemorrhagic Disease Virus
Real-time RT-PCR for the detection of epizootic hemorrhagic disease virus (EHDV) in clinical samples is a fast and sensitive tool for the diagnosis and confirmation of disease. Several real-time RT-PCR methods have been reported over the last 10 years. In this chapter, we describe seven duplex real-time RT-PCR assays to amplify part of genome segment 2Sailleau, Corinne +2 more
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American Journal of Veterinary Research, 1987
SUMMARY Six calves were inoculated with 1 of 2 North American serotypes of epizootic hemorrhagic disease virus (ehdv) and then inoculated with the second serotype 16 weeks later. One calf did not develop an immune response to ehdv after primary inoculation and was removed from the study. Viremia after primary inoculation was transient.
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SUMMARY Six calves were inoculated with 1 of 2 North American serotypes of epizootic hemorrhagic disease virus (ehdv) and then inoculated with the second serotype 16 weeks later. One calf did not develop an immune response to ehdv after primary inoculation and was removed from the study. Viremia after primary inoculation was transient.
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Progress in clinical and biological research, 1985
The degree of relatedness between 4 US serotypes (10, 11, 13 and 17) of bluetongue (BT) virus (BTV) were determined by both oligonucleotide fingerprint analyses of double-stranded (ds) RNA as well as tryptic peptide analyses of viral coded polypeptides.
P, Roy +5 more
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The degree of relatedness between 4 US serotypes (10, 11, 13 and 17) of bluetongue (BT) virus (BTV) were determined by both oligonucleotide fingerprint analyses of double-stranded (ds) RNA as well as tryptic peptide analyses of viral coded polypeptides.
P, Roy +5 more
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Isolation of epizootic hemorrhagic disease virus from sheep in western Turkey
2014Epizootic hemorrhagic disease (EHD) is a systemic viral infection of ruminants, especially deer, characterized by systemic blood circulation disorders and death. Data on the prevalence, clinical and pathological characteristics, and virulence of the infection in sheep are very limited in the literature.
Yavru, S. +4 more
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A serologic investigation of epizootic hemorrhagic disease virus in China between 2014 and 2019
Virologica Sinica, 2022Zhen xing Yang, zhanhong li, Jianbo Zhu
exaly