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EXPERIMENTAL INFECTION IN NORTH AMERICAN ELK WITH EPIZOOTIC HEMORRHAGIC DISEASE VIRUS

Journal of Wildlife Diseases, 1973
Two elk (Cervus canadensis) were inoculated intravenously with epizootic hemorrhagic disease virus. No overt signs of disease were observed in the elk although one animal had a slight febrile response. A cell associated viremia was detected in both elk, which persisted in one animal for 30 days post-inoculation.
Gerald L. Hoff, Daniel O. Trainer
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Epizootic hemorrhagic disease virus serotype 6 experimentation on adult cattle

Research in Veterinary Science, 2013
Epizootic hemorrhagic disease virus (EHDV), an arthropod-borne orbivirus (family Reoviridae), is an emerging pathogen of wild and domestic ruminants closely related to bluetongue virus (BTV). EHDV serotype 6 (EHDV6) has recently caused outbreaks close to Europe in Turkey and Morocco and a recent experimental study performed on calves inoculated with ...
Bréard, Emmanuel   +13 more
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Plaque Neutralization of Bluetongue Virus and Epizootic Hemorrhagic Disease Virus in BHK21 Cells

American Journal of Veterinary Research, 1976
SUMMARY Plaque assay and plaque neutralization of bluetongue virus and epizootic hemorrhagic disease virus were studied in baby hamster kidney (bhk21) cells grown under an overlay containing gum tragacanth. Tests were done in plastic panels, each with 24 wells, and variables were established for achieving reproducible results.
M M, Jochim, S C, Jones
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TRANSMISSION OF TWO STRAINS OF EPIZOOTIC HEMORRHAGIC DISEASE VIRUS IN DEER BY Culicoides variipennis

Journal of Wildlife Diseases, 1977
Two strains of epizootic hemorrhagic disease virus (EHDV), New Jersey (NJ) and Kentucky (KY), of deer were biologically transmitted between white-tailed deer, Odocoileus virginianus, by Culicoides variipennis. The KY strain, isolated from C. variipennis collected during an epizootic in deer, was identified as EHDV by serological tests.
N. M. Foster   +4 more
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Identification of bluetongue virus and epizootic hemorrhagic disease virus serotypes in French Guiana in 2011 and 2012

Veterinary Microbiology, 2014
In French Guiana, the sero- and viro-prevalence of Bluetongue virus (BTV) is high but the circulating serotypes remain unknown. No data are available regarding the prevalence of Epizootic hemorrhagic disease (EHD). This study was conducted to assess the prevalence and to identify the circulating serotypes of these two Orbiviruses in this region (BTV ...
Viarouge, Cyril   +9 more
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No evidence for involvement of sheep in the epidemiology of cattle virulent epizootic hemorrhagic disease virus

Veterinary Microbiology, 2011
Epizootic hemorrhagic disease virus (EHDV) is an Orbivirus. While not previously considered as an important disease in cattle, several EHDV serotypes (EHDV-6 and 7) have recently been implicated in disease outbreaks. The involvement of sheep in the epidemiology of EHDV is still not understood.
S. Levi   +6 more
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Fibrils, Tubules, and Crystals in Cells Infected with Epizootic Hemorrhagic Disease Virus

Proceedings, annual meeting, Electron Microscopy Society of America, 1980
Epizootic hemorrhagic disease virus (EHDV), an orbivirus taxonomically and an arbovirus epidemiologically, replicated in cytoplasmic matrixes of infected cells.1-3 Filaments and macrotubules were observed in the cytoplasm and in association with viroplasms in
Ruth D. Breckon, Neil M. Foster
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Detection of Epizootic Hemorrhagic Disease Virus in Culicoides variipennis (Diptera: Ceratopogonidae)

Journal of Medical Entomology, 1991
Nucleic acid hybridization was used to detect epizootic hemorrhagic disease (EHD) virus serotype 1 and serotype 2 in Culicoides variipennis (Coquillett). Adult females were inoculated intrathoracically with virus, then were assayed daily for the presence of viral RNA for 2 wk, at which time maximum virus replication is likely to occur. Viral RNA of EHD
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Development and statistic evaluation of a plaque assay for the virus of epizootic hemorrhagic disease of deer

Canadian Journal of Microbiology, 1970
A plaque assay system for the virus of epizootic hemorrhagic disease of deer in the WI-2 subline of BHK-21 cells is described. The system uses infection of the cells in suspension before growth as a monolayer under supplemented Leibovitz L-15 medium incorporating gum tragacanth. Crude cell lysates were initially used as a source of virus for the assay
L. W. Macpherson   +3 more
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Epizootic Hemorrhagic Disease Virus Titration

The titration of viruses onto susceptible cell lines is an important virological technique used to quantify infectious viral titers. It forms an integral component of epizootic hemorrhagic disease virus (EHDV) research, including estimating infectivity, calculating multiplicity of infection, and confirming virus propagation in cell culture.
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