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Molecular Serotyping of Epizootic Hemorrhagic Disease Virus
Molecular methods are routinely used for the differential diagnosis and genetic characterization of viral disease of livestock. Real-time, quantitative PCR (qPCR) allows RNA/DNA sequence detection and quantification and is considered the gold standard diagnostic method for most viruses.openaire +2 more sources
Infection of Culicoides Biting Midges with Epizootic Hemorrhagic Disease Virus
Epizootic hemorrhagic disease virus (EHDV) is transmitted by Culicoides biting midges. Studies aiming to predict the likely spread of EHDV require an understanding of the viral infection and replication kinetics within these insects, including the proportion of the insect population that are able to support virus transmission. Here, we describe methodsChristopher, Sanders, Kerry, Newbrook
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Molecular and genetic comparisons of two serotypes of epizootic hemorrhagic disease of deer virus
American Journal of Veterinary Research, 1986SUMMARY The virus-specific double-stranded genome RNA of 2 serotypes of epizootic hemorrhagic disease of deer virus (ehdv) was evaluated by use of coelectrophoresis in polyacrylamide and agarose gel systems. The molecular weights of virion RNA segments were 0.32 to 2.57 × 106 for ehdv-1 and 0.33 to 2.54 × 106 for ehdv-2. Seven of 10 double-stranded RNA
J, Kusari, P, Roy
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American Journal of Veterinary Research, 1988
SUMMARY Epizootic hemorrhagic disease virus was isolated from cattle and sheep in northeastern Colorado during July and August 1984. The isolates were identified as serotype 2 by plaque-inhibition serotyping, genome electropherotyping, and protein analysis.
L H, Thompson +2 more
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SUMMARY Epizootic hemorrhagic disease virus was isolated from cattle and sheep in northeastern Colorado during July and August 1984. The isolates were identified as serotype 2 by plaque-inhibition serotyping, genome electropherotyping, and protein analysis.
L H, Thompson +2 more
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Virus Isolation of Epizootic Hemorrhagic Disease Virus in Cell Culture
Virus isolation is used to assist in the diagnosis and confirmation of viral infections. Successful isolation of a virus is highly dependent upon the quality of starting material. Here we describe the preparation and isolation of epizootic hemorrhagic disease virus (EHDV) from blood and tissue samples in tissue culture flasks (TCFs) through the ...openaire +2 more sources
Whole Genome Sequencing of Epizootic Hemorrhagic Disease Virus
Next-generation sequencing (NGS) technologies are continuously being developed and are becoming a more cost-effective tool for the characterization of viral genomes. Whole genome sequencing of segmented viruses, such as epizootic hemorrhagic disease virus (EHDV), provides insights into the molecular epidemiology as well as such viral evolutionary ...openaire +2 more sources
Epizootic hemorrhagic disease virus infection of type I interferon receptor deficient mice
Veterinary Microbiology, 2012Type I interferon receptor deficient (IFNAR(-/-)) mice were infected with an Israeli isolate of serotype 7 of epizootic hemorrhagic disease virus (EHDV; Orbivirus, Reoviridae). Two out of two mice that received 5×10(5) 50% tissue culture infectious doses (TCID(50)) by intraperitoneal injection died or were euthanized in a moribund state on day 5 after ...
Michael Eschbaumer +3 more
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Real-Time RT-PCR Assays for Typing of Epizootic Hemorrhagic Disease Virus
Real-time RT-PCR for the detection of epizootic hemorrhagic disease virus (EHDV) in clinical samples is a fast and sensitive tool for the diagnosis and confirmation of disease. Several real-time RT-PCR methods have been reported over the last 10 years. In this chapter, we describe seven duplex real-time RT-PCR assays to amplify part of genome segment 2Sailleau, Corinne +2 more
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American Journal of Veterinary Research, 1987
SUMMARY Six calves were inoculated with 1 of 2 North American serotypes of epizootic hemorrhagic disease virus (ehdv) and then inoculated with the second serotype 16 weeks later. One calf did not develop an immune response to ehdv after primary inoculation and was removed from the study. Viremia after primary inoculation was transient.
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SUMMARY Six calves were inoculated with 1 of 2 North American serotypes of epizootic hemorrhagic disease virus (ehdv) and then inoculated with the second serotype 16 weeks later. One calf did not develop an immune response to ehdv after primary inoculation and was removed from the study. Viremia after primary inoculation was transient.
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Cervical cancer prevention and control in women living with human immunodeficiency virus
Ca-A Cancer Journal for Clinicians, 2021Philip E Castle, Vikrant V Sahasrabuddhe
exaly