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Whole Genome Sequencing of Epizootic Hemorrhagic Disease Virus.
Methods in molecular biologyNext-generation sequencing (NGS) technologies are continuously being developed and are becoming a more cost-effective tool for the characterization of viral genomes. Whole genome sequencing of segmented viruses, such as epizootic hemorrhagic disease virus (EHDV), provides insights into the molecular epidemiology as well as such viral evolutionary ...
Simon King
semanticscholar +3 more sources
Virus Isolation of Epizootic Hemorrhagic Disease Virus in Cell Culture.
Methods in molecular biologyVirus isolation is used to assist in the diagnosis and confirmation of viral infections. Successful isolation of a virus is highly dependent upon the quality of starting material. Here we describe the preparation and isolation of epizootic hemorrhagic disease virus (EHDV) from blood and tissue samples in tissue culture flasks (TCFs) through the ...
Amanda Corla
semanticscholar +3 more sources
Infection of Culicoides Biting Midges with Epizootic Hemorrhagic Disease Virus.
Methods in molecular biologyEpizootic hemorrhagic disease virus (EHDV) is transmitted by Culicoides biting midges. Studies aiming to predict the likely spread of EHDV require an understanding of the viral infection and replication kinetics within these insects, including the proportion of the insect population that are able to support virus transmission. Here, we describe methods
Christopher Sanders, Kerry Newbrook
semanticscholar +3 more sources
Real-Time RT-PCR Assays for Typing of Epizootic Hemorrhagic Disease Virus.
Methods in molecular biologyReal-time RT-PCR for the detection of epizootic hemorrhagic disease virus (EHDV) in clinical samples is a fast and sensitive tool for the diagnosis and confirmation of disease. Several real-time RT-PCR methods have been reported over the last 10 years. In this chapter, we describe seven duplex real-time RT-PCR assays to amplify part of genome segment 2
C. Sailleau +2 more
semanticscholar +4 more sources
Real-Time RT-PCR for the Diagnosis of Epizootic Hemorrhagic Disease Virus.
Methods in molecular biologyReal-time reverse transcription-polymerase chain reaction (real-time RT-PCR) has become an essential tool in rapid and reliable detection of animal diseases such as epizootic hemorrhagic disease (EHD). Here we provide a protocol for the detection of epizootic hemorrhagic disease virus (EHDV) genetic material in blood and tissue samples, using a real ...
Martin Ashby
semanticscholar +3 more sources
Epizootic Hemorrhagic Disease Virus: Methods and Protocols
Methods in Molecular Biologysemanticscholar +2 more sources
Hemagglutination of epizootic hemorrhagic disease virus
Archives of Virology, 1981Hemagglutination of epizootic hemorrhagic disease virus (EHDV) with a variety of erythrocyte species at 4 degrees C, room temperature and 37 degrees C was dependent on the NaCl molarity and the pH of the diluent. The hemagglutination inhibition test was used to identify EHDV serotypes.
S, Tokuhisa +6 more
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Purification and stability of epizootic hemorrhagic disease virus
Journal of Virological Methods, 1982Purification of epizootic hemorrhagic disease virus (EHDV) from sonicated cell culture supernatant was effected using polyethylene glycol (PEG) precipitation and two CsCl density gradient centrifugations. During purification procedures the pH was maintained at values close to pH 8.0 as pH stability for EHDV was maximal between pH 7.2 and pH 9.0. During
R, Lewanczuk, T, Yamamoto
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Experimental infection of calves with epizootic hemorrhagic disease virus
American Journal of Veterinary Research, 1999Abstract Objective To determine whether experimental inoculation with a field strain of epizootic hemorrhagic disease virus serotype-2 (EHDV-2) suspected of causing clinical disease in naturally infected cattle would cause clinical disease in calves. Animals 8 calves.
M J, Abdy +2 more
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