Results 171 to 180 of about 1,517 (201)

Quantification of Ergovaline in Tall Fescue by Near Infrared Reflectance Spectroscopy

Crop Science, 1997
Near infrared (NIR) reflectance spectroscopy was examined as a possible alternative to high performance liquid chromatography (HPLC) analysis for ergovaline in tall fescue (Festuca arundinacea Schreber). Tall fescue samples were collected from field plots in Missouri and analyzed by NIR spectroscopy and HPLC.
C. A. Roberts, R. E. Joost, G. E. Rottinghaus   +2 more
openaire   +1 more source

Seasonal Fluctuation of Ergovaline and Total Ergot Alkaloid Concentrations in Tall Fescue Regrowth

Crop Science, 2011
ABSTRACTCommon cultivars of tall fescue [Schedonorus arundinaceus (Schreb.) Dumort = Lolium arundinaceum (Schreb.) Darbysh.] host a fungal endophyte that produces ergot alkaloids. These alkaloids are linked to fescue toxicosis, a serious livestock disorder in the United States.
Wendi M. Rogers, Craig A. Roberts, John G. Andrae, David K. Davis, George E. Rottinghaus, Nicholas S. Hill, Robert L. Kallenbach, Don E. Spiers   +7 more
openaire   +1 more source

Ergovaline does not alter the severity of ryegrass staggers induced by lolitrem B

New Zealand Veterinary Journal, 2018
To investigate a possible interaction between lolitrem B and ergovaline by comparing the incidence and severity of ryegrass staggers in sheep grazing ryegrass (Lolium perenne) containing lolitrem B or ryegrass containing both lolitrem B and ergovaline.Ninety lambs, aged approximately 6 months, were grazed on plots of perennial ryegrass infected with ...
Sarah c Finch, Wade J Mace, Chikako van Koten   +2 more
exaly   +3 more sources

Temperature-dependent responses of rats to ergovaline derived from endophyte-infected tall fescue

Journal of Animal Science, 1995
Ergovaline, found in Acremonium coenophialum-infected tall fescue, is considered to be responsible for many symptoms associated with fescue toxicosis. Rats were tested to determine time-related thermoregulatory responses to acute treatment with ergovaline during specific thermal challenges. Isolated ergovaline was administered to rats (15 micrograms/kg
D E, Spiers, Q, Zhang, P A, Eichen, G E, Rottinghaus, G B, Garner, M R, Ellersieck   +5 more
openaire   +2 more sources

Improved Extraction and HPLC Methods for Ergovaline from Plant Material and Rumen Fluid

Journal of Veterinary Diagnostic Investigation, 1994
Ergovaline, the main ergopeptine alkaloid produced in tall fescue infected with Acremonium coenophialum, is known to cause tall fescue toxicosis. Current methods in use for quantifying ergovaline in plant material have several disadvantages, including large solvent volumes and long analysis time.
A M, Cràig, D, Bilich, J T, Hovermale, R E, Welty   +3 more
openaire   +2 more sources

DETERMINATION OF ERGOVALINE IN ENDOPHYTED SEEDS BY HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY (HPTLC)

Journal of Liquid Chromatography & Related Technologies, 2000
High performance thin layer chromatography (HPTLC) is used to rapidly determine the presence and amount of ergovalin in seeds of Graminaceae infested with Neotyphodium Coenophialum. Quinine is used as an internal standard. The mycotoxin is solubilized under acid conditions and extracted with chloroform under alkaline conditions.
Videmann, B., Bony, Sylvie, Berny, Philippe   +2 more
openaire   +2 more sources

Ergovaline-induced vasoconstriction in an isolated bovine lateral saphenous vein bioassay.

Journal of animal science, 2007
Ergovaline has been proposed as a toxic component of endophyte-infected tall fescue. As many of the symptoms of fescue toxicosis are a result of compromised circulation, the objective of this study was to examine the vasoconstrictive potentials of ergovaline and a more documented ergopeptine, ergotamine, using a bovine, lateral (cranial branch ...
J L, Klotz, L P, Bush, D L, Smith, W D, Shafer, L L, Smith, B C, Arrington, J R, Strickland   +6 more
openaire   +1 more source

Bioaccumulation of ergovaline in bovine lateral saphenous veins in vitro.

Journal of animal science, 2009
Ergot alkaloids have been associated with vasoconstriction in grazing livestock affected by the fescue toxicosis syndrome. Previous in vitro investigations studying how ergot alkaloids caused vasoconstriction have shown that ergovaline has a distinct receptor affinity and sustained contractile response. A similar contractile response has not been noted
J L, Klotz, B H, Kirch, G E, Aiken, L P, Bush, J R, Strickland   +4 more
openaire   +1 more source

Thermoregulatory Effects of Ergovaline Isolated from Endophyte-Infected Tall Fescue Seed on Rats

Journal of Agricultural and Food Chemistry, 1994
Ergovaline (EV) was isolated from Acremonium coenophialum-infected tall fescue seed. Seed (2 kg) was extracted with 20 L of 5% lactic acid in water/methanol (4:1), filtered, and passed over an Amberlite XAD-2 column. Ergovaline was eluted with MeOH, concentrated, and chromatographed on two reversed-phase C 18 columns (purity >95%, 40% overall recovery)
Qinggen Zhang, Donald E. Spiers, George E. Rottinghaus, George B. Garner   +3 more
openaire   +1 more source

ERGOVALINE TOXICITY ON CACO-2 CELLS AS ASSESSED BY MTT, ALAMARBLUE, AND DNA ASSAY

In Vitro Cellular and Developmental Biology-Animal, 2003
The exact mechanisms of fescue toxicity in animals have yet to be established, but it has been associated with an inability to thrive. Ergovaline is the major ergopeptine alkaloid associated with fungal infections of tall fescue. Gastrointestinal (GI) toxicity of ergovaline (10(-11) to 10(-4) M) was evaluated in Caco-2 cells (mimicking the GI ...
openaire   +2 more sources