Results 111 to 120 of about 4,040,528 (306)

Error propagation in estimate of p3D.

open access: yes, 2019
The fractional random error, , and the systematic error, Δp3D, in the p3D estimate increase with mis-measurement of cell perimeter, but are not significantly influenced by neglecting small cell areas.
Tristan A. Sharp (6289376)   +3 more
core   +1 more source

Structural and biochemical analysis of a B12 superbinder

open access: yesFEBS Letters, EarlyView.
BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices   +3 more
wiley   +1 more source

Estimation for the Multivariate Errors-in-Variables Model with Estimated Error Covariance Matrix

open access: yesThe Annals of Statistics, 1984
The authors consider the estimation problem of multivariate errors-in- variables models. Let \(r\times 1\) row vectors \(y_ i\) and \(k\times 1\) row vectors \(x_ i\) satisfy \(y_ i=\beta_ 0+x_ i\beta\), \(i=1,2,...,n\), where \(\beta_ 0\) and \(\beta\) are 1\(\times r\) and \(k\times r\) matrices of parameters, respectively.
Amemiya, Yasuo, Fuller, Wayne A.
openaire   +3 more sources

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

open access: yesFEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge   +6 more
wiley   +1 more source

A natural boundary element method for the Sobolev equation in the 2D unbounded domain

open access: yesBoundary Value Problems, 2017
In this article, we devote ourselves to establishing a natural boundary element (NBE) method for the Sobolev equation in the 2D unbounded domain. To this end, we first constitute the time semi-discretized super-convergence format for the Sobolev equation
Fei Teng, Zhendong Luo, Jing Yang
doaj   +1 more source

Prospecting the protein design landscape

open access: yesFEBS Letters, EarlyView.
This review outlines the current state of various protein design approaches. We discuss the current possibilities enabled by recently released tools, highlight future avenues to pursue in protein design, and underscore the crucial role of key databases and resources for successful protein design workflows.
Jakob R. Riccabona   +4 more
wiley   +1 more source

L‐aspartate oxidase provides new insights into fumarate reduction in anaerobic darkness in Synechocystis sp. PCC6803

open access: yesFEBS Letters, EarlyView.
Synechocystis strains deficient in succinate dehydrogenase (SDH) secrete more succinate than the WT under dark anaerobic conditions, supporting that SDH then primarily acts as SDH, not as a fumarate reductase. L‐aspartate oxidase (Laspo) from Synechocystis is functional under anaerobic conditions, reducing fumarate to succinate.
Kateryna Kukil   +3 more
wiley   +1 more source

ERROR ESTIMATION AND ADAPTIVITY IN ELASTOPLASTICITY

open access: yesInternational Journal for Numerical Methods in Engineering, 1996
Summary: A method is developed to control the parameters of a finite element computation for time-dependent material models. To evaluate discretization errors, we use a global error measure in constitutive relation based on Drucker's inequality. This error includes, over the studied time interval, the error of the finite element model and the error of ...
Gallimard, L.   +2 more
openaire   +3 more sources

The ubiquitin system in normal and infected germinal center B cells

open access: yesFEBS Letters, EarlyView.
The ubiquitin system plays a central role in germinal center (GC) B cells, influencing differentiation to long‐lived memory B cells. Oncogenic gammaherpesviruses gain access to memory B cells by establishing latency in GC B cells. Mapping ubiquitin mechanisms in normal and infected GC B cells will define specific molecular circuits in B cells germane ...
Destiny Davis   +2 more
wiley   +1 more source

DNA ligase LigE increases bacterial transformation with damaged extracellular DNA

open access: yesFEBS Letters, EarlyView.
The DNA ligase LigE from the naturally competent Gram‐negative bacterium Neisseria gonorrhoaea increases transformation with cut extracellular DNA. This process is ATP‐dependent and relies on the catalytic activity of LigE; it also requires that LigE contains an intact periplasmic localisation sequence, indicating that ligE functions in the periplasm ...
Jolyn Pan   +4 more
wiley   +1 more source

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