Results 191 to 200 of about 7,716 (265)

[Chronic pain and persistent pain: Conceptual and clinical differences in pain terminology]. [PDF]

Aten Primaria
Vázquez Canales LM, Perelló-Bratescu A, Domínguez-García M, Morral Fernández A.   +3 more
europepmc   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

[Informal caregiving in primary care: Who cares for the caregivers?] [PDF]

Aten Primaria
Bordes Capolat IR.
europepmc   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

From phenotypic to molecular diagnosis: Insights from a clinical immunology service focused on inborn errors of immunity in Colombia [PDF]

Biomedica
Fernandes Pineda M, Zea-Vera AF.
europepmc   +1 more source

Heterotropic regulation and negative homotropic cooperativity

FEBS Open Bio, EarlyView.
We identified a structural module common to some proteins that couple negative cooperativity with heterotropic regulation, two features that rarely coexist. These proteins are ring‐like and present an ordered asymmetry whereby noncontacting subunits are symmetric, and their tertiary structure differs from that of contacting subunits.
Veronica Morea, Francesco Angelucci, Federica Arnesano, Gianmarco Pascarella, Patrizio Di Micco, Andrea Bellelli   +5 more
wiley   +1 more source

Immunization errors of COVID-19 vaccines in Minas Gerais. [PDF]

Rev Bras Enferm
Ferreira WV, Andrade TIX, Barbosa TCP, Santos RCD, Oliveira SH, Guimarães VC, Guimarães EAA, Oliveira VC.   +7 more
europepmc   +1 more source

Identification and characterisation of calcitonin receptor isoforms expressed in glioblastoma derived glioma stem and U‐87 MG cells

FEBS Open Bio, EarlyView.
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta, Sebastian GB Furness, Tahereh Gharbi, Ric De Paoli‐Iseppi, Shweta S. Joshi, Michael Clark, David L. Hare, Peter Wookey   +7 more
wiley   +1 more source

Independent assessment of the WHO Skin Neglected Tropical Diseases application for leprosy detection. [PDF]

Rev Panam Salud Publica
Deps P, Amorim BBC, Repsold T, Almonfrey D, do Espírito Santo RB, Loureiro RM, Enechukwu NA, Barreiro TZ, Rodrigues MM, Fonseca AMF, Lima GN, Florian MC, Ruiz-Postigo JA.   +12 more
europepmc   +1 more source