Results 101 to 110 of about 492,895 (317)

Dynamics of immobilized and native Escherichia coli dihydrofolate reductase by quasielastic neutron scattering

, 2006
The internal dynamics of native and immobilized Escherichia coli dihydrofolate reductase (DHFR) have been examined using incoherent quasielastic neutron scattering.
Smith, J.C., Ollivier, Jacques, Kurkal, V., Monk, C., Oettl, M., Finney, J.L., Tehei, Moeava, Monk, Colin R., Kurkal, Vandana, Smith, Jeremy C., Tehei, M., Finney, John L., Ollivier, J., Oettl, Martin, Daniel, R.M., Daniel, Roy M.   +15 more
core   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Studien zur Proteintranslokation in Escherichia coli : Untersuchung der Membranproteine SecYEG und YidC unter Verwendung biochemischer und kristallographischer Methoden

, 2008
Transport of proteins into or across cellular membranes is mediated by the conserved and ubiquitous Sec-machinery. The Sec-homologue in the inner membrane of Escherichia coli is SecYEG.
Lotz, Mirko
core  

Structural insights into the dynamics and function of the C-terminus of the E. coli RNA chaperone Hfq

, 2011
The hexameric Escherichia coli RNA chaperone Hfq (Hfq(Ec)) is involved in riboregulation of target mRNAs by small trans-encoded RNAs. Hfq proteins of different bacteria comprise an evolutionarily conserved core, whereas the C-terminus is variable in ...
Beich-Frandsen, Mads (Department of Structural and Computational Biology, Centre for Molecular Biology, University of Vienna), Kontaxis, Georg (Department of Structural and Computational Biology, Centre for Molecular Biology, University of Vienna), Blasi, U., Rajkowitsch, L., Miles, Andrew (Birkbeck College), Sjöblom, Björn (Department of Structural and Computational Biology, Centre for Molecular Biology, University of Vienna), Miles, Andrew J., Svergun, D.I., Kloiber, K., Kristina Djinović-Carugo, Vecerek, B., Karin Kloiber, Konarev, P.V., Lukas Rajkowitsch, Hämmerle, Hermann Konrad (Department of Biochemistry and Cell Biology, Centre for Molecular Biology, University of Vienna), Georg Kontaxis, Bläsi, Udo (Department of Microbiology, Immunobiology and Genetics, Centre for Molecular Biology, University of Vienna), Djinovic-Carugo, K., Robert Konrat, Branislav Večerek, Konarev, Peter V. (EMBL Hamburg), Andrew J. Miles, Kloiber, Karin (Department of Structural and Computational Biology, Centre for Molecular Biology, University of Vienna), Rajkowitsch, Lukas (Department of Biochemistry and Cell Biology, Centre for Molecular Biology, University of Vienna), Udo Bläsi, Wallace, Bonnie A., Svergun, D. I., Petr V. Konarev, Svergun, Dimitri I. (EMBL Hamburg), Björn Sjöblom, Hammerle, H., Mads Beich-Frandsen, Wallace, B. A., Sjoblom, B., Miles, A. J., Konrat, Robert (Department of Structural and Computational Biology, Centre for Molecular Biology, University of Vienna), Vecerek, Branislav (Department of Microbiology, Immunobiology and Genetics, Centre for Molecular Biology, University of Vienna), Dimitri I. Svergun, B. A. Wallace, Beich Frandsen, M., Djinovic Carugo, K., Konarev, P. V., Beich-Frandsen, M., Konrat, R., Djinovic-Carugo, Kristina (Department of Structural and Computational Biology, Centre for Molecular Biology, University of Vienna), Hermann Hämmerle, Kontaxis, G.   +46 more
core   +1 more source

The structure of the Escherichia coli phosphotransferase IIAmannitol reveals a novel fold with two conformations of the active site [PDF]

, 1998
Background: The bacterial phosphoenolpyruvate-dependent phosphotransferase system (PTS) catalyses the cellular uptake and subsequent phosphorylation of carbohydrates.
van Montfort, R.L M, Dijkstra, B.W., Dijkstra, Bauke W., Robillard, G.T., Kalk, Kor H., Kouwijzer, Milou L.C.E.,, Pijning, Tjaard, van Montfort, Rob LM, van Montfort, Robert, Montfort, Rob L.M. van,, Dijkstra, Bauke W.,, Robillard, George T.,, Kalk, Kor H, Pijning, T., Kouwijzer, Milou LCE, Hanqyi, I., Robillard, George T, Dijkstra, Bauke W, Kalk, K.H., Pijning, Tjaard,, Pijning, Tjaard; id_orcid, Kouwijzer, Marie, Kalk, Kor H.,, Hangyi, Ilona,, Robillard, George T., Hangyi, Ilona, Kouwijzer, M.L C E   +26 more
core   +1 more source

The crystal structure of the Borrelia burgdorferi nicotinamidase BBE22 resolves a long‐standing annotation error

FEBS Open Bio, EarlyView.
The crystal structure of Borrelia burgdorferi nicotinamidase (PncA/BBE22) reveals the correct full‐length protein initiated from a non‐canonical AUU start codon. The structure validates previous biochemical findings and resolves a long‐standing annotation error, demonstrating that the truncated database sequence is structurally incompatible with the ...
Kalvis Brangulis
wiley   +1 more source

Novel AlkB Dioxygenases – Alternative Models for In Silico and In Vivo Studies [PDF]

, 2012
Background: ALKBH proteins, the homologs of Escherichia coli AlkB dioxygenase, constitute a direct, single-protein repair system, protecting cellular DNA and RNA against the cytotoxic and mutagenic activity of alkylating agents, chemicals significantly ...
Mielecki, Marcin, Marcin Grynberg, Zugaj, Dorota Łucja, Anna Muszewska, Jadwiga Nieminuszczy, Mielecki Marcin, Marcin Grynberg (183714), Marcin Mielecki (186643), Mielecki, Damian, Jan Piwowarski (186640), Dorota Ł. Zugaj (186635), Mielecki Damian, Piwowarski Jan, Grzesiuk Elżbieta, Grzesiuk, Elzbieta, Marcin Mielecki, Nieminuszczy, Jadwiga, Zugaj Dorota Ł., Damian Mielecki (186634), Chojnacka, Aleksandra, Grynberg Marcin, Dorota Ł Zugaj, Grynberg, Marcin, Nieminuszczy Jadwiga, Jan Piwowarski, Muszewska Anna, Jadwiga Nieminuszczy (186644), Muszewska, Anna, Aleksandra Chojnacka (186642), Piwowarski, Jan, Chojnacka Aleksandra, Damian Mielecki, Aleksandra Chojnacka, Elżbieta Grzesiuk (186645), Elżbieta Grzesiuk, Anna Muszewska (186638)   +35 more
core   +1 more source

Molecular characterization of covRS mutations in M1UK Streptococcus pyogenes

FEBS Open Bio, EarlyView.
Group A Streptococcus (GAS) acquires covRS mutations driving a hypervirulent bacterial state, frequently associated with invasive disease‐like necrotizing fasciitis. We demonstrate that the newly emerged M1UK GAS lineage can also acquire these mutations.
Jarrad Pritchard, Emma‐Jayne Proctor, Jiawa Wang, Nikolas P. Johnston, Andrew Hayes, Georgia McCorkell, Haibo Yu, Jai Tree, Mark R. Davies, Mark J. Walker, Ronald Sluyter, Stephan Brouwer, Martina L. Sanderson‐Smith   +12 more
wiley   +1 more source

Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA

FEBS Open Bio, EarlyView.
PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro, Fernando Lucas Palhano   +1 more
wiley   +1 more source

Structural genomics of human proteins – target selection and generation of a public catalogue of expression clones

Microbial Cell Factories, 2005
Background The availability of suitable recombinant protein is still a major bottleneck in protein structure analysis. The Protein Structure Factory, part of the international structural genomics initiative, targets human proteins for structure ...
Harttig Ulrich, Sievert Volker, Scheich Christoph, Büssow Konrad, Schultz Jörg, Simon Bernd, Bork Peer, Lehrach Hans, Heinemann Udo   +8 more
doaj   +1 more source