Results 81 to 90 of about 1,964,169 (255)

Rainbow Vectors for Broad-Range Bacterial Fluorescence Labeling.

PLoS ONE, 2016
Since their discovery, fluorescent proteins have been widely used to study protein function, localization or interaction, promoter activity and regulation, drug discovery or for non-invasive imaging.
Mariette Barbier, F Heath Damron
doaj   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

Mutation rate plasticity in rifampicin resistance depends on Escherichia coli cell–cell interactions [PDF]

, 2014
Variation of mutation rate at a particular site in a particular genotype, in other words mutation rate plasticity (MRP), can be caused by stress or ageing. However, mutation rate control by other factors is less well characterized.
John A. D. Aston, Aston, Elizabeth, Christopher G. Knight, Roman V. Belavkin, Knight, Christopher G., Aston, JA, Aston, John A D, Rash, BM, Knight, C. G. (Christopher G.), Elizabeth Aston, Channon, A, Krašovec, Rok; id_orcid, Knight, CG, An Kadirvel, Aston, John A. D., Forbes, S, Alastair Channon, Channon, Alastair, Knight, Christopher G.; id_orcid, Aston, E, Krašovec, Rok, Belavkin, RV, Forbes, Sarah, Aston, John A.D., Sarah Forbes, Kadirvel, Manikandan, Belavkin, Roman V., Bharat M. Rash, Kadirvel, Manikandan; id_orcid, Rash, Bharat M., Krašovec, R, Kadirvel, M, Channon, A., Belavkin, R., Krašovec, R., Aston, E., Forbes, S., Kadirvel, M., Rash, B., Knight, C., Aston, J.   +40 more
core   +1 more source

Data on low-molecular weight proteins of Escherichia coli treated by essential oils components, tetracycline, chlorine and peroxide by MALDI-TOF MS

Data in Brief, 2018
In this dataset we provide MALDI-TOF MS spectra of Escherichia coli. The data presented in this article are related to the article entitled “Stress response of Escherichia coli to essential oil components – insights on low-molecular-weight proteins from ...
Matěj Božik, Pavel Cejnar, Petr Maršík, Pavel Klouček   +3 more
doaj   +1 more source

Remarkable diversity in the enzymes catalyzing the last step in synthesis of the pimelate moiety of biotin. [PDF]

PLoS ONE, 2012
Biotin synthesis in Escherichia coli requires the functions of the bioH and bioC genes to synthesize the precursor pimelate moiety by use of a modified fatty acid biosynthesis pathway.
Madelyn M Shapiro, Vandana Chakravartty, John E Cronan   +2 more
doaj   +1 more source

Lipopolysaccharide uptake is augmented in lipopolysaccharide‐tolerant mouse macrophage‐like cells via increased CD14 expression

FEBS Open Bio, EarlyView.
In normal (nontolerant) cells, CD14 is crucial for both LPS uptake and LPS signaling. In LPS‐tolerant cells, in which LPS‐induced TNF‐α and IFN‐β production is suppressed, there is a dramatic increase in surface CD14 expression. The overexpressed CD14 in LPS‐tolerant cells is responsible for the enhanced LPS uptake without inducing pro‐inflammatory ...
Saeka Nishihara, Takayuki Manabe, Mika Jouta, Kiyoshi Kawasaki   +3 more
wiley   +1 more source

Posttranslational Modifications of Ribosomal Proteins in Escherichia coli

Acta Naturae, 2011
А number of ribosomal proteins inEscherichia coliundergo posttranslational modifications. Six ribosomal proteins are methylated (S11, L3, L11, L7/L12, L16, and L33), three proteins are acetylated (S5, S18, and L7), and protein S12 is methylthiolated. Extra amino acid residues are added to protein S6. С-terminal amino acid residues are partially removed
Nesterchuk, M., Sergiev, P., Dontsova, O.   +2 more
openaire   +3 more sources

Structure of the Cytoplasmic Loop between Putative Helices II and III of the Mannitol Permease of Escherichia coli: A Tryptophan and 5-Fluorotryptophan Spectroscopy Study [PDF]

, 2008
In this work, four single tryptophan (Trp) mutants of the dimeric mannitol transporter of Escherichia coli, EIImtl, are characterized using Trp and 5-fluoroTrp (5-FTrp) fluorescence spectroscopy.
Bokhove, Marcel,, Broos, Jaap,, Erwin P. P. Vos, Hesp, Ben H., Vos, Erwin P. P., Vos, Erwin P.P.,, Hesp, Ben H.,, Broos, Jaap, Bokhove, Marcel, Ben H. Hesp, Jaap Broos, Marcel Bokhove   +11 more
core   +2 more sources

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Consequences of Membrane Protein Overexpression in Escherichia coli

Molecular & Cellular Proteomics, 2007
Overexpression of membrane proteins is often essential for structural and functional studies, but yields are frequently too low. An understanding of the physiological response to overexpression is needed to improve such yields. Therefore, we analyzed the consequences of overexpression of three different membrane proteins (YidC, YedZ, and LepI) fused to
Wagner, Samuel, Baars, Louise, Ytterberg, A. Jimmy, Klussmeier, Anja, Wagner, Claudia S., Nord, Olof, Nygren, Per-Åke, van Wijk, Klaas J., de Gier, Jan-Willem   +8 more
openaire   +3 more sources