Results 71 to 80 of about 1,964,169 (255)

Engineering peptides into antibodies—opportunities and strategies for therapeutic innovation

FEBS Letters, EarlyView.
Peptides and antibodies occupy complementary therapeutic niches. Peptides recognize difficult targets in a compact format, while antibodies add specificity, long half‐life, and effector functions. This review examines strategies that merge both modalities—peptide grafting into loops, terminal and Fc fusions, and bioconjugation—highlighting how ...
Jinling Wang, Ruslana Romaniuk, Hristo L. Svilenov   +2 more
wiley   +1 more source

Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network

FEBS Letters, EarlyView.
Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley   +1 more source

Dynamics of immobilized and native Escherichia coli dihydrofolate reductase by quasielastic neutron scattering [PDF]

, 2006
The internal dynamics of native and immobilized Escherichia coli dihydrofolate reductase (DHFR) have been examined using incoherent quasielastic neutron scattering.
Smith, J.C., Ollivier, Jacques, Kurkal, V., Monk, C., Oettl, M., Finney, J.L., Tehei, Moeava, Monk, Colin R., Kurkal, Vandana, Smith, Jeremy C., Tehei, M., Finney, John L., Ollivier, J., Oettl, Martin, Daniel, R.M., Daniel, Roy M.   +15 more
core   +1 more source

Anethole Attenuates Enterotoxigenic Escherichia coli-Induced Intestinal Barrier Disruption and Intestinal Inflammation via Modification of TLR Signaling and Intestinal Microbiota

Frontiers in Microbiology, 2021
This study aimed to investigate the effects of dietary anethole supplementation on the growth performance, intestinal barrier function, inflammatory response, and intestinal microbiota of piglets challenged with enterotoxigenic Escherichia coli K88 ...
Qingyuan Yi, Jiaxin Liu, Yufeng Zhang, Hanzhen Qiao, Fang Chen, Fang Chen, Shihai Zhang, Shihai Zhang, Wutai Guan, Wutai Guan   +9 more
doaj   +1 more source

Functional comparison of EncB and EncC cargo proteins in iron storage within the Myxococcus xanthus encapsulin

FEBS Letters, EarlyView.
Encapsulins are protein nanocompartments that play an important role in iron storage. In the Myxococcus xanthus encapsulin system, two cargo proteins called EncB and EncC contribute to iron mineralization. Here, we show that EncB and EncC generate iron‐containing minerals with distinct chemical compositions, suggesting that the composition of stored ...
Harry B. McDowell, Thomas Walther, Egbert Hoiczyk   +2 more
wiley   +1 more source

Genetic distribution, characterization, and function of Escherichia coli type III secretion system 2 (ETT2)

iScience
Summary: Many Gram-negative bacteria use type Ⅲ secretion system (T3SS) to inject effector proteins and subvert host signaling pathways, facilitating the growth, survival, and virulence.
Xinyu Wang, Hong Zhu, Jiangang Hu, Beibei Zhang, Weiqi Guo, Zhiyang Wang, Di Wang, Jingjing Qi, Mingxing Tian, Yanqing Bao, Fusheng Si, Shaohui Wang   +11 more
doaj   +1 more source

L‐aspartate oxidase provides new insights into fumarate reduction in anaerobic darkness in Synechocystis sp. PCC6803

FEBS Letters, EarlyView.
Synechocystis strains deficient in succinate dehydrogenase (SDH) secrete more succinate than the WT under dark anaerobic conditions, supporting that SDH then primarily acts as SDH, not as a fumarate reductase. L‐aspartate oxidase (Laspo) from Synechocystis is functional under anaerobic conditions, reducing fumarate to succinate.
Kateryna Kukil, Jeffrey A. Hawkes, Cecilia Blikstad, Pia Lindberg   +3 more
wiley   +1 more source

IMPDH inhibition enhances cytarabine efficacy in SAMHD1‐expressing leukaemia cells via guanine nucleotide depletion

Molecular Oncology, EarlyView.
Cytarabine is a key therapy for acute myeloid leukaemia (AML), but its efficacy is limited by the dNTPase SAMHD1, which hydrolyses its active metabolite. Screening nucleotide biosynthesis inhibitors revealed that IMPDH inhibitors selectively sensitise SAMHD1‐proficient AML cells to cytarabine.
Miriam Yagüe‐Capilla, Christopher Dirks, Caroline Eiden, Sonja K. Fesenmayer, Femke M. Hormann, Yolande Klootsema, Ingrid Lilienthal, Si Min Zhang, Nikolas Herold, Sean G. Rudd   +9 more
wiley   +1 more source

Comparison of bacterial intracellular and secreted proteins produced in milk versus medium for Escherichia coli by proteomic analysis

Journal of Dairy Science
: The growth and reproduction of microorganisms are dependent on nutrient supply. Here, milk and Luria-Bertani (LB) media were used as nutrition sources for Escherichia coli, and the changes in bacterial and secretory proteins at 3 time points (3, 9, and
Qiyue Sun, Yanxin Li, Hongning Jiang, Qijing Du, Jun Wang, Rongbo Fan, Rongwei Han, Yongxin Yang   +7 more
doaj   +1 more source

Proteomic analysis of Escherichia coli detergent-resistant membranes (DRM).

PLoS ONE, 2019
Membrane microdomains or lipid rafts compartmentalize cellular processes by laterally organizing membrane components. Such sub-membrane structures were mainly described in eukaryotic cells, but, recently, also in bacteria.
José E Guzmán-Flores, Lidia Steinemann-Hernández, Luis E González de la Vara, Marina Gavilanes-Ruiz, Tony Romeo, Adrián F Alvarez, Dimitris Georgellis   +6 more
doaj   +1 more source