Preparation of Soluble Proteins fromEscherichia coli [PDF]
Current Protocols in Protein Science, 1995 AbstractPurification of human IL‐1β is used in this unit as an example of the preparation of a soluble protein fromE. coli. Bacteria containing IL‐1β are lysed, and IL‐1 β in the resulting supernatant is purified by anion‐exchange chromatography, salt precipitation, and cation‐exchange chromatography, and then concentrated.
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Bacteria‐Responsive Nanostructured Drug Delivery Systems for Targeted Antimicrobial Therapy
Advanced Materials, EarlyView.Bacteria‐responsive nanocarriers are designed to release antimicrobials only in the presence of infection‐specific cues. This selective activation ensures drug release precisely at the site of infection, avoiding premature or indiscriminate release, and enhancing efficacy.
Guillermo Landa +3 more
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Processing of in vitro-synthesized gag precursor proteins of human immunodeficiency virus (HIV) type 1 by HIV proteinase generated in Escherichia coli [PDF]
, 1988 Hans‐Georg Kräusslich +4 more
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AI–Guided 4D Printing of Carnivorous Plants–Inspired Microneedles for Accelerated Wound Healing
Advanced Materials, EarlyView.This work presents an artificial intelligence (AI)‐guided 4D‐printed microneedle platform inspired by carnivorous plants for wound healing. A thermo‐responsive shape memory polymer enables body temperature–triggered self‐coiling for autonomous wound closure.
Hyun Lee +21 more
wiley +1 more source
A novel plant E3 ligase stabilizes Escherichia coli heat shock factor σ32
Scientific Reports, 2017 The heat shock response is crucial for organisms against heat-damaged proteins and maintaining homeostasis at a high temperature. Heterologous expression of eukaryotic molecular chaperones protects Escherichia coli from heat stress.
Yulong Niu +8 more
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Advanced Materials Interfaces, EarlyView.We generate hafnium nanostructures by first depositing polystyrene particles of varying diameter followed by physical vapour deposition of hafnium thin films. The resultant structure shows a kill‐on‐contact effect against both Gram‐negative Escherichia coli and Gram‐positive Staphylococcus aureus.
Andrew Boden +6 more
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RNA BiologyProtein aggregation, a consequence of misfolding and impaired proteostasis, can lead to cellular malfunctions such as various proteinopathies.
Chan Park +6 more
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\u3cem\u3ePlasmodium falciparum\u3c/em\u3e SSB Tetramer Binds Single-Stranded DNA Only in a Fully Wrapped Mode [PDF]
, 2012 The tetrameric Escherichia coli single-stranded DNA (ssDNA) binding protein (Ec-SSB) functions in DNA metabolism by binding to ssDNA and interacting directly with numerous DNA repair and replication proteins.
Antony, Edwin +3 more
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Advanced Materials Interfaces, EarlyView.Zero‐dimensional carbon nanomaterials are presented as multifunctional platforms linking structure, property, and sensing performance. Surface engineering and heteroatom doping modulate electron‐transfer and luminescent behavior, enabling electrochemical, photoluminescent, and electrochemiluminescent detection. Fundamental design principles, analytical
Gustavo Martins +8 more
wiley +1 more source
Expression in Escherichia coli of the flavin and the haem domains of Hansenula anomala flavocytochrome b2 (flavodehydrogenase and b2 core) and characterization of the recombinant proteins¶ [PDF]
, 1993 Maria Chiara Silvestrini +4 more
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