Results 221 to 230 of about 729,912 (260)

Ribosomal protein pool of Escherichia coli

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1970
Abstract Escherichia coli B cells, which were grown in a heavy medium ( 15 NH 4 Cl and 2 H 2 O) and then transferred to a light medium ( 14 NH 4 Cl and 1 H 2 O), contained an expanded pool of ribosomal proteins. The expansion was attributable to the partial breakdown of ribosomes formed in the heavy medium due to the density-transfer and the ...
Daisuke Nakada, Robert M. Young
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Induction of protein X in Escherichia coli

Molecular and General Genetics MGG, 1977
Certain treatments that damage DNA and/or inhibit replication in E. coli have been reported to induce synthesis of a new protein, termed protein X, in recA+ lexA+ strains. We have examined some of the treatments that might induce protein X and we have, in particular, tested the hypothesis of Gudas and Pardee (1975) that DNA degradation products play an
John W. Little, Philip C. Hanawalt
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Overproduction of Escherichia coli NusA protein

Gene, 1983
The nusA gene of Escherichia coli has been cloned into the plasmid vector pKC30 under the control of the inducible lambda pL promoter. When a strain carrying this plasmid is induced, NusA protein is overproduced more than 100-fold and constitutes 20-30% of the total cellular protein.
Richard R. Burgess, Peter O. Olins, Bruce D. Erickson   +2 more
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Incorporation of fluorotryptophans into proteins of Escherichia coli

Biochemistry, 1975
A tryptophan-requiring strain of Escherichia coli can go through two doublings of optical density after L-tryptophan is replaced in the medium by 4-fluorotryptophan, during which the fluoro analog displaces approximately 75% of the L-tryptophan in cell protein.
Chien Ho, E. A. Pratt
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PROTEIN SECRETION IN ESCHERICHIA COLI

Annual Review of Microbiology, 1985
INTRO DUCT I ON . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 615 MODELS OF PROTEIN E XPORT ..... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . .. .... . .. . ...
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Strategies for protein coexpression in Escherichia coli

Nature Methods, 2006
E. coli is a convenient host for heterologous protein expression. Its advantages include high levels of heterologous gene expression and scalability of experiments, low cost, fast growth, a lack of posttranslational modification and an ability to express labeled (isotope or seleno-methionine) proteins.
Leemor Joshua-Tor, Niraj H. Tolia
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Membrane Protein Production in Escherichia coli

2020
Escherichia coli is the workhorse of the structural biology lab. In addition to routine cloning and molecular biology, E. coli can be used as a factory for the production of recombinant membrane proteins. Purification of homogeneous samples of membrane protein expressed in E. coli is a significant bottleneck for researchers, and the protocol we present
Ali A. Kermani, Benjamin C. McIlwain
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Recombinant protein expression in Escherichia coli [PDF]

Current Opinion in Biotechnology, 1999
Escherichia coli is one of the most widely used hosts for the production of heterologous proteins and its genetics are far better characterized than those of any other microorganism. Recent progress in the fundamental understanding of transcription, translation, and protein folding in E.
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Recombinant protein secretion in Escherichia coli

Biotechnology Advances, 2005
The secretory production of recombinant proteins by the Gram-negative bacterium Escherichia coli has several advantages over intracellular production as inclusion bodies. In most cases, targeting protein to the periplasmic space or to the culture medium facilitates downstream processing, folding, and in vivo stability, enabling the production of ...
Gabriel A. Monteiro, D. K. Summers, Filipe Mergulhão   +2 more
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Escherichia coli Repressor Proteins

1989
Genetic regulation is an essential function in all living organisms. In prokaryotes genetic control provides responsivity to a constantly changing external milieu, and bacterial systems have proved extremely valuable in elucidating the variety of potential mechanisms.
Kyle L. Wick, Kathleen S. Matthews
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