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Escherichia coli Repressor Proteins
1989Genetic regulation is an essential function in all living organisms. In prokaryotes genetic control provides responsivity to a constantly changing external milieu, and bacterial systems have proved extremely valuable in elucidating the variety of potential mechanisms.
Kyle L. Wick, Kathleen S. Matthews
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Periplasmic Targeting of Recombinant Proteins in Escherichia coli
2007Targeting recombinant protein production to the periplasmic space of Escherichia coli presents several advantages over cytoplasmic production in inclusion bodies and at the same time overcomes the low productivity problem often associated with culture medium secretion.
Filipe J, Mergulhão +1 more
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Fractionation of the ribosomal protein from Escherichia coli
Journal of Molecular Biology, 1964The proteins isolated from purified 70 s ribosomes of Escherichia coli have been studied in 6 to 8 M-urea by starch gel electrophoresis and by fractionation on carboxymethyl-cellulose columns. Evidence now exists for the presence of at least 24 different starch gel bands, each of which we believe represents a specific protein.
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Overproduction of Heterologous Proteins in Escherichia coli
2020H, Yasueda, H, Matsui
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Protein diffusion in Escherichia coli
2009This dissertation describes the creation of a system to provide insight about the affects of protein expression on intracellular diffusion. Fluorescence recovery after photobleaching (FRAP) is used to obtain diffusion coefficients. Nuclear magnetic resonance spectroscopy detection of 19F isotopic-enriched proteins and fluorescent labeling with FlAsH ...
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