Results 321 to 330 of about 762,581 (360)

Membrane Protein Production in Escherichia coli

2020
Escherichia coli is the workhorse of the structural biology lab. In addition to routine cloning and molecular biology, E. coli can be used as a factory for the production of recombinant membrane proteins. Purification of homogeneous samples of membrane protein expressed in E. coli is a significant bottleneck for researchers, and the protocol we present
Benjamin C, McIlwain, Ali A, Kermani
openaire   +2 more sources

Recombinant protein secretion in Escherichia coli

Biotechnology Advances, 2005
The secretory production of recombinant proteins by the Gram-negative bacterium Escherichia coli has several advantages over intracellular production as inclusion bodies. In most cases, targeting protein to the periplasmic space or to the culture medium facilitates downstream processing, folding, and in vivo stability, enabling the production of ...
F J M, Mergulhão, D K, Summers, G A, Monteiro   +2 more
openaire   +2 more sources

PROTEIN SECRETION IN ESCHERICHIA COLI

Annual Review of Microbiology, 1985
INTRO DUCT I ON . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 615 MODELS OF PROTEIN E XPORT ..... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . .. .... . .. . ...
openaire   +2 more sources

Escherichia coli Repressor Proteins

1989
Genetic regulation is an essential function in all living organisms. In prokaryotes genetic control provides responsivity to a constantly changing external milieu, and bacterial systems have proved extremely valuable in elucidating the variety of potential mechanisms.
Kyle L. Wick, Kathleen S. Matthews
openaire   +1 more source

Recombinant protein expression in Escherichia coli

Current Opinion in Biotechnology, 1999
Escherichia coli is one of the most widely used hosts for the production of heterologous proteins and its genetics are far better characterized than those of any other microorganism. Recent progress in the fundamental understanding of transcription, translation, and protein folding in E.
openaire   +2 more sources

Hyperproduction of araC protein from Escherichia coli

Biochemistry, 1982
Hypersynthesis of araC protein from Escherichia coli has been accomplished. The araC gene was cloned on plasmid pBR322, and some of the noncoding DNA preceding the araC gene was removed by exonuclease digestion. Finally, a DNA fragment containing the lac promoter and ribosome binding site was placed in front the araC gene.
R F, Schleif, M A, Favreau
openaire   +2 more sources

DNA Replication Proteins of Escherichia Coli

Annual Review of Biochemistry, 1978
PERSPECTIVES AND SUMMARY ..... .. .. 1163 ESCHERICHIA COLI CHROMOSOME REPLICATION ........ .. ..... ...... .... 116S In Vivo DNA Replication 1165 In Vitro DNA Replication 1166 SINGLE-STRANDED CIRCULAR DNA-DEPENDENT DNA SYNTHESIS .... 1168 Priming of Single-Stranded DNA Synthesis ..
openaire   +2 more sources

Extracellular recombinant protein production from Escherichia coli

Biotechnology Letters, 2009
Escherichia coli is the most commonly used host for recombinant protein production and metabolic engineering. Extracellular production of enzymes and proteins is advantageous as it could greatly reduce the complexity of a bioprocess and improve product quality.
Ye, Ni, Rachel, Chen
openaire   +2 more sources

[Protein kinase activity in Escherichia coli].

Comptes rendus des seances de l'Academie des sciences. Serie D, Sciences naturelles, 1979
When growing E. coli in a minimal medium, at least four proteins from the soluble fraction and one ribosome-associated protein are found phosphorylated at the level of their threonine and serine residues.
Manai, M., Cozzone, A.
openaire   +2 more sources

Induction of protein X in Escherichia coli

Molecular and General Genetics MGG, 1977
Certain treatments that damage DNA and/or inhibit replication in E. coli have been reported to induce synthesis of a new protein, termed protein X, in recA+ lexA+ strains. We have examined some of the treatments that might induce protein X and we have, in particular, tested the hypothesis of Gudas and Pardee (1975) that DNA degradation products play an
J W, Little, P C, Hanawalt
openaire   +2 more sources