Results 351 to 360 of about 1,293,873 (407)

Protein export in Escherichia coli

Current Opinion in Biotechnology, 1992
The export of protein from Escherichia coli has been studied by genetic, biochemical and biophysical techniques. These studies have defined a number of steps in the export pathway and have identified the cellular components required for the translocation process. New information is presented on the function of some of these components.
K, Johnson, C K, Murphy, J, Beckwith
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The Escherichia coli SeqA protein

Plasmid, 2009
The Escherichia coli SeqA protein contributes to regulation of chromosome replication by preventing re-initiation at newly replicated origins. SeqA protein binds to new DNA which is hemimethylated at the adenine of GATC sequences. Most of the cellular SeqA is found complexed with the new DNA at the replication forks.
Torsten, Waldminghaus, Kirsten, Skarstad
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Protein secretion pathways in Escherichia coli

Current Opinion in Biotechnology, 1994
The export of proteins to the Escherichia coli periplasm is a well established system for heterologous protein production. With a better understanding of the protein export (SecA, Y-dependent) process and a greater awareness of the conditions necessary for correct folding of proteins in the periplasm, serious efforts are now being made to manipulate ...
M A, Blight, C, Chervaux, I B, Holland
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Ribosomal protein pool of Escherichia coli

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1970
Abstract Escherichia coli B cells, which were grown in a heavy medium ( 15 NH 4 Cl and 2 H 2 O) and then transferred to a light medium ( 14 NH 4 Cl and 1 H 2 O), contained an expanded pool of ribosomal proteins. The expansion was attributable to the partial breakdown of ribosomes formed in the heavy medium due to the density-transfer and the ...
R M, Young, D, Nakada
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Overproduction of Escherichia coli NusA protein

Gene, 1983
The nusA gene of Escherichia coli has been cloned into the plasmid vector pKC30 under the control of the inducible lambda pL promoter. When a strain carrying this plasmid is induced, NusA protein is overproduced more than 100-fold and constitutes 20-30% of the total cellular protein.
P O, Olins, B D, Erickson, R R, Burgess
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Ribosomal protein pools in Escherichia coli

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1969
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Marchis-Mouren, G., Cozzone, A., Marvaldi, J.   +2 more
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Membrane Protein Production in Escherichia coli

2020
Escherichia coli is the workhorse of the structural biology lab. In addition to routine cloning and molecular biology, E. coli can be used as a factory for the production of recombinant membrane proteins. Purification of homogeneous samples of membrane protein expressed in E. coli is a significant bottleneck for researchers, and the protocol we present
Benjamin C, McIlwain, Ali A, Kermani
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Recombinant protein secretion in Escherichia coli

Biotechnology Advances, 2005
The secretory production of recombinant proteins by the Gram-negative bacterium Escherichia coli has several advantages over intracellular production as inclusion bodies. In most cases, targeting protein to the periplasmic space or to the culture medium facilitates downstream processing, folding, and in vivo stability, enabling the production of ...
F J M, Mergulhão, D K, Summers, G A, Monteiro   +2 more
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PROTEIN SECRETION IN ESCHERICHIA COLI

Annual Review of Microbiology, 1985
INTRO DUCT I ON . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 615 MODELS OF PROTEIN E XPORT ..... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .. . . . . . . . . . . . . .. .... . .. . ...
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Escherichia coli Repressor Proteins

1989
Genetic regulation is an essential function in all living organisms. In prokaryotes genetic control provides responsivity to a constantly changing external milieu, and bacterial systems have proved extremely valuable in elucidating the variety of potential mechanisms.
Kyle L. Wick, Kathleen S. Matthews
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