Results 171 to 180 of about 55,913 (214)

Penetrating the biofilm barrier: characterization of <i>Escherichia</i> phage vB_EcoS-TPF103dw and harnessing depolymerase to combat Shiga toxin-producing <i>Escherichia coli</i> O103 biofilm. [PDF]

Front Microbiol
Campos DL, Liao YT, Harden LA, Zhang Y, Wu VCH.   +4 more
europepmc   +1 more source

Generation and modulation of catalytically relevant states of a dye‐decolourizing peroxidase using time‐resolved serial femtosecond crystallography with drop‐on‐chip mixing and X‐ray‐driven reduction

Acta Crystallographica Section D, Volume 82, Issue 5, Page 434-445, May 2026.
We characterize the formation of a ferryl state of a dye‐decolourizing peroxidase by drop‐on‐chip serial femtosecond crystallography and also the modulation of the heme redox state using dose‐resolved serial synchrotron crystallography.Metalloenzymes containing a heme cofactor catalyse a wide range of oxidative reactions critical to life. Understanding
Marina Lučić, Johan Glerup, Pierre Aller, Danny Axford, Nicholas Devenish, Jaehyun Park, Anastasiia Shilova, Arturo Landeros de la Isla, Richard W. Strange, Tiankun Zhou, Robin L. Owen, Jonathan A. R. Worrall, Michael A. Hough   +12 more
wiley   +1 more source

Roles of the membrane-binding motif and the C-terminal domain of RNase E in localization and diffusion in <i>E. coli</i>. [PDF]

Elife
Troyer L, Wang YH, Shobhna S, Kim S, Ramsey B, Woo J, Tajkhorshid E, Kim S.   +7 more
europepmc   +1 more source

Structures of variants of Escherichia coli flavodiiron‐type nitric oxide reductase reveal changes in the di‐iron site

Acta Crystallographica Section D, Volume 82, Issue 5, Page 457-470, May 2026.
Flavodiiron proteins (FDPs) are di‐iron enzymes that reduce NO and/or O2 with distinct substrate selectivity. We have produced E. coli FDP variants targeting second coordination‐sphere residues and determined their structures. Although the kinetics remained unchanged, the E.
Patrícia T. Borges, Filipe Folgosa, Maria C. Martins, Guillaume Gotthard, Peter van der Linden, Philippe Carpentier, Miguel Teixeira, Carlos Frazão, Célia V. Romão   +8 more
wiley   +1 more source

Bacillus subtilis MutL samples multiple conformations during nucleotide binding and hydrolysis. [PDF]

Structure
Rodríguez González J, Davis CL, Wilkins H, Erie DA, Guarné A.   +4 more
europepmc   +1 more source

Optimal 1TEL–target protein linker character is target protein‐dependent

Acta Crystallographica Section D, Volume 82, Issue 5, Page 516-532, May 2026.
In this study, we examine the effect of short to medium‐length flexible, semi‐flexible and rigid linkers on the crystallization of a DARPin or the TNK1 UBA domain fused to the 1TEL protein crystallization chaperone, demonstrating that while rigid linkers can impair crystallization and reduce diffraction quality, the ideal linker character remains ...
Maria J. Pedroza Romo, Alihikaua Keliiliki, Jacob C. Averett, Joseph F. Gonzalez, Ethan Noakes, Elijah W. Wilson, Conrad Smith, Blake Averett, Dalton Hansen, Riley Nickles, Miles Bradford, Sara Soleimani, Tobin Smith, Supeshala Nawarathnage, Prasadika Samarwickrama, Ariel Kelsch, Derick Bunn, Cameron Stewart, Wisdom Abiodun, Evan Tsubaki, Seth Brown, Tzanko I. Doukov, James D. Moody   +22 more
wiley   +1 more source

Radiation‐induced peroxide rupture and its temperature‐dependent repair probed by homogeneous X‐ray irradiation

Acta Crystallographica Section D, Volume 82, Issue 5, Page 492-503, May 2026.
This manuscript investigates the X‐ray‐induced radiolysis of the catalytic C5‐peroxide adduct in urate oxidase crystals, utilizing a top‐hat X‐ray beam to monitor peroxide occupancy across extensive dose series at both 100 K and room temperature. The study reveals a kinetic phase transition where the peroxide decays rapidly via zero‐order kinetics at ...
Symeon Koulas, Soi Bui, Julius B. Kirkegaard, Gleb Bourenkov, Roberto A. Steiner   +4 more
wiley   +1 more source

The effects of intravenous immunoglobulin on intestinal inflammation: a systematic review of animal and clinical studies. [PDF]

PeerJ
Chu T, Yuan X, Wang T, Zhang W, Yan X, Li C, Jiang P, Ye S, Ma L, Sun P, Du X, Fu P, Wang Z.   +12 more
europepmc   +1 more source

Cryo‐EM reveals ArnA contamination during purification of a ciliary protein complex

Acta Crystallographica Section D, Volume 82, Issue 5, Page 404-410, May 2026.
ArnA contamination was independently observed during the purification of a soluble, low‐yield protein complex, leading to an unintended 3.23 Å resolution cryo‐EM structure. This work broadens recent findings and highlights ArnA as a recurrent His‐tag‐associated contaminant in Escherichia coli purification workflows.Endogenous Escherichia coli proteins ...
Xuguang Jiang, Masahide Kikkawa
wiley   +1 more source

Nucleotide signals coordinate activation and inhibition of bacterial immunity. [PDF]

Nature
Yamaguchi S, Fernandez SG, Wassarman DR, Lüders M, Schwede F, Kranzusch PJ.   +5 more
europepmc   +1 more source