Results 131 to 140 of about 2,330,903 (349)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

BIOFUEL: ALTERNATIVE FOR APPLICATIONS IN OXI-COMBUSTION THERMAL PROCESSES

open access: yesNonconventional Technologies Review
Bioenergy, as a form of regenerable power, is an excellent alternative to ensure a clean and healthy environment and at the same time, to preserve natural energy resources that are constantly decreasing.
Lucian Paunescu   +2 more
doaj  

ẢNH HƯỞNG CỦA VIỆC BỔ SUNG SORBITOL VÀ ETHANOL ĐẾN SỰ THAY ĐỔI ĐỘ HOẠT ĐỘNG CỦA NƯỚC VÀ CHẤT LƯỢNG KHÔ CÁ SẶC RẰN

open access: yesTạp chí Khoa học Đại học Cần Thơ, 2009
ảnh hưởng của việc bổ sung sorbitol và ethanol riêng lẻ cũng như sự kết hợp đồng thời hai chất tan này đến sự thay đổi hoạt độ của nước trong khô cá sặc rằn được tiến hành.
Trần Thanh Trúc   +2 more
doaj  

The excess wing in the dielectric loss of glass-forming ethanol: A relaxation process

open access: yes, 2000
A detailed dielectric investigation of liquid, supercooled liquid, and glassy ethanol reveals a third relaxation process, in addition to the two processes already known.
Brand, R.   +3 more
core   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

open access: yesFEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake   +4 more
wiley   +1 more source

KINETIKA TRANSESTERIFIKASI BIODIESEL JARAK PAGAR

open access: yesTeknik, 2012
Biodiesel were produced by trans-etherification of castor oil with alcohol in the presence of NaOH catalyst. Thereaction mechanism and model of castor oil trans-etherification isA + 3B C + 3 DA, B, C, and D were castor oil, alcohol, glycerol, and ester ...
Buchori Luqman, Budi Sasongko Setia
doaj  

Construction Cost Sensitivity of a Lignocellulosic Ethanol Biorefinery [PDF]

open access: yes
The technology has been developed to convert feedstock with cellulose content into ethanol. However, ethanol produced from cellulosic feedstock is the same as ethanol distilled from grain. The objective of research is to determine the price per gallon of
Busby, David P.   +2 more
core   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

open access: yesFEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee   +6 more
wiley   +1 more source

Long‐term hippocampal alterations and cognitive impairment in a murine model of surgical sepsis

open access: yesFEBS Open Bio, EarlyView.
Using a mouse model of surgical sepsis, we tested long‐term memory and analyzed the transcriptome of single cells isolated from the hippocampus. Survivor mice showed worse memory, loss of certain brain cell subpopulations, and abnormal immune cell activity—suggesting that post‐sepsis brain alterations may be linked to cognitive deficits.
Dong Seong Cho   +4 more
wiley   +1 more source

Early‐life high‐fat diet exposure increases Achilles tendon stiffness and induces transcriptomic alterations

open access: yesFEBS Open Bio, EarlyView.
Early‐life exposure to a high‐fat diet altered intact Achilles tendons in rat offspring, making them thinner, stiffer, and molecularly distinct even without injury. These findings suggest that developmental high‐fat diet exposure may impair tendon quality and increase susceptibility to mechanical overload or tendon injury later in life.
Heyong Yin   +3 more
wiley   +1 more source

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