Results 81 to 90 of about 791,104 (305)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Differential penetration of ethanol and water in Si-chabazite: High pressure dehydration of azeotrope solution [PDF]

, 2019
This study is aimed to shed light on the mechanisms at the basis of the differential penetration of alcohol and water in hydrophobic zeolites at ambient (Pamb) and non-ambient pressure.
Arletti R., Giorgia, Confalonieri, Giovanna, Vezzalini, Quartieri S., Rossella, Arletti, Fois E., Ettore, Fois, Simona, Quartieri, Daou T. J., T. Jean, Daou, gloria, tabacchi, Confalonieri G., Vezzalini G., Tabacchi G.   +13 more
core   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Medial prefrontal cortical extracellular dopamine responses after acutely experimenter-administered or orally self-administered ethanol

, 2013
textDopamine signaling in the prefrontal cortex is thought to play a role in ethanol abuse. However, little is known about how ethanol affects dopamine signaling in the region.
Schier, Christina Joanne
core  

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

Anodic Dissolution of Aluminum in KOH-Ethanol Solutions

Journal of Electrochemistry, 2004
The feasibility of employing aluminum in KOH ethanol solution as a battery anode system is discussed. Weight loss measurement showed that its corrosion rate is low enough to be used in sealed batteries. Through anodic polarization, EIS and galvanostatic discharge, it was found that Al can keep electrochemical activity in a wide potential range in this ...
H.B. Shao, J.M. Wang, X.Y. Wang, J.Q. Zhang, C.N. Cao   +4 more
openaire   +1 more source

Preparation and characterization of vinyltriethoxysilane (VTES) modified silicalite-1/PDMS hybrid pervaporation membrane and its application in ethanol separation from dilute aqueous solution

, 2010
To improve the affinity between silicalite-1 and polydimethylsiloxane (PDMS), the silicalite-1 particles were modified by a silane coupling agent vinyltriethoxysilane (VTES) and incorporated into polydimethylsiloxane (PDMS) matrix for the preparation of ...
Yi, Shouliang, Su, Yi, Wan, YH, Wan, Yinhua   +3 more
core   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

An optimized method for Oil Red O staining with the salicylic acid ethanol solution. [PDF]

Adipocyte, 2023
Du J, Zhao L, Kang Q, He Y, Bi Y.
europepmc   +1 more source

Environmental and social dimensions of fuel ethanol production in Cradock, South Africa, in the context of the wider biofuels debate

, 2013
Liquid biofuels are fossil fuel replacements in the form of fuel ethanol and biodiesel. Advocates of biofuels highlight their potential to mitigate climate change from reduced greenhouse gas emissions and socio-economic benefits for countries that ...
Nasterlack, Tobias
core