Results 91 to 100 of about 241,979 (317)

Torsion and bending of nucleic acids studied by subnanosecond time-resolved fluorescence depolarization of intercalated dyes [PDF]

, 1982
Subnanosecond time‐resolved fluorescence depolarization has been used to monitor the reorientation of ethidium bromide intercalated in native DNA, synthetic polynucleotide complexes, and in supercoiled plasmid DNA.
Millar, D. P., Robbins, R. J., Zewail, A. H.   +2 more
core  

Inhalable Artificial Polymeric Nucleases Degrading Neutrophil Extracellular Trap‐DNAs and Alleviating Pulmonary Fibrosis

Advanced Science, EarlyView.
Pulmonary fibrosis (PF) is promoted by NETs that induce fibroblast activation. PEG‐PIm, a polymeric artificial DNase is reported that efficiently degrades NET‐DNA via phosphodiester bond hydrolysis. Inhalation of PEG‐PIm in a mouse PF model prevents fibroblast activation and fibrosis progression, offering a promising therapeutic strategy for NET ...
Yibo Du, Chenxu Zhu, Ruifeng Wang, Shi Chen, Chuang Li, Defang OuYang, Lixin Liu, Yongming Chen   +7 more
wiley   +1 more source

Investigation of Gel Electrophoresis Stains

Al-SalamJournal for Medical Science
Gel electrophoresis is essential in molecular biology and biochemistry for separating and analyzing DNA, RNA, and proteins. UV sensitivity and compatibility have led to the use of ethidium bromide stains.
ibrahim ramzi hadi, Dilara Bozdogan, Ali Ahmed Kamal Khedher Agha, Ruaa Hussein Ali, Safa Al-Shattawi   +4 more
doaj   +1 more source

Independence from kinetoplast DNA maintenance and expression is associated with multi-drug resistance in Trypanosoma brucei in vitro [PDF]

, 2014
It is well known that several antitrypanosomatid drugs accumulate in the parasite's mitochondrion, where they often bind to the organellar DNA, the kinetoplast.
A. Schnaufer, Agbe, Bruhn, Brun, Cristodero, Dean, Deterding, Herbert, Hoare, Holmes, Kaminsky, Lai, Lanteri, M. K. Gould, Mathis, Mukherjee, Painter, Priest, Roy Chowdhury, Räz, Schnaufer, Schnaufer, Schnaufer, Shapiro, Stuart, Tang, Thuita, Ward, Wenzler, Wenzler, Wilson, Zhang, Zuma   +32 more
core   +1 more source

Super‐Resolution Compatible DNA Labeling Technique Reveals Chromatin Mobility and Organization Changes During Differentiation

Advanced Science, EarlyView.
A novel chromatin‐labeling probe is developed to investigate chromatin mobility during differentiation. The silicon rhodamine–conjugated dCTP is efficiently delivered into iPSCs and NSCs using a synthetic transporter. Super‐resolution microscopy and single‐particle tracking show decreased chromatin mobility during differentiation.
Maruthi K. Pabba, Miroslav Kuba, Tomáš Kraus, Kerem Celikay, Janis Meyer, Sunil Kumar Pradhan, Andreas Maiser, Hartmann Harz, Heinrich Leonhardt, Karl Rohr, Michal Hocek, M. Cristina Cardoso   +11 more
wiley   +1 more source

A cryptic targeting signal creates a mitochondrial FEN1 isoform with tailed R-Loop binding properties.

PLoS ONE, 2013
A growing number of DNA transacting proteins is found in the nucleus and in mitochondria, including the DNA repair and replication protein Flap endonuclease 1, FEN1. Here we show a truncated FEN1 isoform is generated by alternative translation initiation,
Lawrence Kazak, Aurelio Reyes, Jiuya He, Stuart R Wood, Gloria Brea-Calvo, Torgeir T Holen, Ian J Holt   +6 more
doaj   +1 more source

Application of the SwitchSense Technique for the Study of Small Molecules' (Ethidium Bromide and Selected Sulfonamide Derivatives) Affinity to DNA in Real Time. [PDF]

J Phys Chem B, 2022
Ramotowska S, Spisz P, Brzeski J, Ciesielska A, Makowski M.   +4 more
europepmc   +1 more source

Templated mineralization by charge-modified cowpea mosaic virus [PDF]

, 2013
Templated mineralization of virus particles provides routes to narrowly dispersed nanoparticles that are not readily prepared by other means. The templated mineralization of metal or metal oxide on the external surface of wild-type cowpea mosaic virus ...
Aljabali, Alaa A A, Evans, David J.
core   +1 more source

GDF15 Ameliorates Deoxynivalenol‐Induced Anemia by Resolving Ribosomal Stress–Mediated Erythropoietic Arrest

Advanced Science, EarlyView.
Deoxynivalenol induces anemia by disrupting hematopoietic homeostasis and impairing erythroid lineage commitment and differentiation via ribosomal stress–mediated G1/S cell cycle arrest. GDF15 supplementation alleviates this arrest through the β‐catenin–Myc–p21 signaling axis, highlighting GDF15 as a potential therapeutic target for DON‐induced ...
Yan Li, Peijun Jia, Jingxin Zhang, Huan Zhang, Bing Li, Jiawei Chang, Yating Li, Longzhen Zhao, Yazhe Zhen, Tingting Zheng, Yuanlin Xu, Xiuli An, Shijie Zhang   +12 more
wiley   +1 more source

Fluorometric determination of ethidium bromide efflux kinetics in Escherichia coli

Journal of Biological Engineering, 2009
Background Efflux pump activity has been associated with multidrug resistance phenotypes in bacteria, compromising the effectiveness of antimicrobial therapy.
Monteiro Gabriel A, de Carvalho Carla CCR, Fernandes Pedro, Martins Marta, Couto Isabel, Rodrigues Liliana, Paixão Laura, Sansonetty Filipe, Amaral Leonard, Viveiros Miguel   +9 more
doaj   +1 more source